Plumbridge T W, Brown J R
Biochim Biophys Acta. 1977 Dec 14;479(4):441-9. doi: 10.1016/0005-2787(77)90037-5.
The changes in the visible absorption spectrum and the degree of fluorescence polarization of daunomycin and mepacrine on binding to DNA have been compared with those shown by the archetypical intercalating agent ethidium. The changes are qualitatively similar for the three drugs, namely a bathochromic shift and hypochromicity (giving an isosbestic point) and an increase in polarization of fluorescence when irradiated with polarized light. These effects are typical of intercalation. The effect of changing the nucleic acid conformation has been investigated by repeating the studies with poly(I-C) (a nucleic acid in the A conformation). Upon interaction with poly(I-C) only ethidium shows effects typical of intercalation, neither daunomycin nor mepacrine intercalates into poly(I-C) helix.
已将柔红霉素和米帕林与DNA结合时可见吸收光谱和荧光偏振度的变化，与典型的嵌入剂乙锭所显示的变化进行了比较。这三种药物的变化在性质上是相似的，即红移和减色（产生等吸收点），以及用偏振光照射时荧光偏振度增加。这些效应是嵌入作用的典型特征。通过用聚肌胞苷酸（一种A构象的核酸）重复研究，研究了改变核酸构象的影响。与聚肌胞苷酸相互作用时，只有乙锭显示出典型的嵌入作用效应，柔红霉素和米帕林均不嵌入聚肌胞苷酸螺旋。
