用单克隆抗体对微量培养板中沙眼衣原体包涵体进行间接免疫荧光染色。
Indirect immunofluorescence staining of Chlamydia trachomatis inclusions in microculture plates with monoclonal antibodies.
作者信息
Zapata M, Chernesky M, Mahony J
出版信息
J Clin Microbiol. 1984 Jun;19(6):937-9. doi: 10.1128/jcm.19.6.937-939.1984.
Abstract
Indirect immunofluorescence (IF) staining, using a monoclonal antibody, detected two- to fourfold more inclusions than did iodine staining. Of 274 clinical specimens, 53 (19.3%) were positive by IF on passage 1 as compared with 33 (12%) by iodine staining (P less than 0.005). IF-stained inclusions in McCoy cells in the bottom of microculture wells were readily viewed with a long-focal-length objective at a magnification of 250 X.
摘要
使用单克隆抗体的间接免疫荧光（IF）染色检测到的包涵体比碘染色多两到四倍。在274份临床标本中，第1代时IF检测呈阳性的有53份（19.3%），而碘染色为33份（12%）（P小于0.005）。在微量培养孔底部的 McCoy 细胞中经IF染色的包涵体，用长焦距物镜在250倍放大倍数下很容易观察到。
相似文献
1
Indirect immunofluorescence staining of Chlamydia trachomatis inclusions in microculture plates with monoclonal antibodies.用单克隆抗体对微量培养板中沙眼衣原体包涵体进行间接免疫荧光染色。
J Clin Microbiol. 1984 Jun;19(6):937-9. doi: 10.1128/jcm.19.6.937-939.1984.
2
Detection of Chlamydia trachomatis inclusions in Mccoy cell cultures with fluorescein-conjugated monoclonal antibodies.使用荧光素偶联单克隆抗体检测 McCoy 细胞培养物中的沙眼衣原体包涵体。
J Clin Microbiol. 1983 Apr;17(4):666-8. doi: 10.1128/jcm.17.4.666-668.1983.
3
Sensitivity of immunofluorescence with monoclonal antibodies for detection of Chlamydia trachomatis inclusions in cell culture.用单克隆抗体进行免疫荧光检测细胞培养中沙眼衣原体包涵体的敏感性。
J Clin Microbiol. 1982 Jul;16(1):4-7. doi: 10.1128/jcm.16.1.4-7.1982.
4
Staining characteristics of six commercially available monoclonal immunofluorescence reagents for direct diagnosis of Chlamydia trachomatis infections.六种市售单克隆免疫荧光试剂用于直接诊断沙眼衣原体感染的染色特性。
J Clin Microbiol. 1988 Sep;26(9):1735-7. doi: 10.1128/jcm.26.9.1735-1737.1988.
5
Ortho enzyme immunoassay versus McCoy cell monolayers stained by iodine or fluorescent antibody for detection of Chlamydia trachomatis.用于检测沙眼衣原体的正交酶免疫测定法与经碘或荧光抗体染色的 McCoy 细胞单层法的比较。
J Clin Microbiol. 1990 Jul;28(7):1647-8. doi: 10.1128/jcm.28.7.1647-1648.1990.
6
Comparison of iodine- and fluorescein-labeled monoclonal antibodies for detection of Chlamydia trachomatis inclusions in cells grown in glass vials.
Diagn Microbiol Infect Dis. 1984 Jan;2(1):17-22. doi: 10.1016/0732-8893(84)90019-1.
7
A comparison of the sensitivity of immunofluorescence and Giemsa for staining Chlamydia trachomatis inclusions in cycloheximide-treated McCoy cells.免疫荧光法与吉姆萨染色法对环己酰亚胺处理的 McCoy 细胞中沙眼衣原体包涵体染色敏感性的比较。
J Clin Pathol. 1980 Feb;33(2):177-9. doi: 10.1136/jcp.33.2.177.
8
Evaluation of a genus reactive monoclonal antibody in rapid identification of Chlamydia trachomatis by direct immunofluorescence.通过直接免疫荧光法评估一种属反应性单克隆抗体在沙眼衣原体快速鉴定中的应用。
Genitourin Med. 1985 Aug;61(4):252-4. doi: 10.1136/sti.61.4.252.
9
Comparison of Giemsa and fluorescent monoclonal antibody staining of inoculated cell cultures for diagnosis of Chlamydia trachomatis.
Pathology. 1994 Apr;26(2):194-7. doi: 10.1080/00313029400169461.
10
Early detection of chlamydial inclusions combining the use of cycloheximide-treated McCoy cells and immunofluorescence staining.结合使用环己酰亚胺处理的 McCoy 细胞和免疫荧光染色法对衣原体包涵体进行早期检测。
J Clin Microbiol. 1977 Sep;6(3):285-92. doi: 10.1128/jcm.6.3.285-292.1977.
引用本文的文献
1
Presence of a neutralizing domain in isolates of rubella virus in Cordoba, Argentina.阿根廷科尔多瓦风疹病毒分离株中存在中和结构域。
Clin Diagn Lab Immunol. 1997 Jul;4(4):493-5. doi: 10.1128/cdli.4.4.493-495.1997.
2
Recent advances in Chlamydia trachomatis.沙眼衣原体的最新进展
Eur J Epidemiol. 1985 Dec;1(4):235-56. doi: 10.1007/BF00237099.
3
Effect of blind passage and multiple sampling on recovery of Chlamydia trachomatis from urogenital specimens.盲传和多次采样对泌尿生殖系统标本中沙眼衣原体回收率的影响。
J Clin Microbiol. 1986 Dec;24(6):1029-33. doi: 10.1128/jcm.24.6.1029-1033.1986.
4
Comparison of cell culture with two direct Chlamydia tests using immunofluorescence or enzyme-linked immunosorbent assay.
Eur J Clin Microbiol. 1986 Oct;5(5):569-72. doi: 10.1007/BF02017707.
5
Comparison of a fluorescent monoclonal antibody assay and a tissue culture assay for routine detection of infections caused by Chlamydia trachomatis.
Eur J Clin Microbiol. 1986 Oct;5(5):554-8. doi: 10.1007/BF02017704.
6
Accuracy of immunoglobulin M immunoassay for diagnosis of chlamydial infections in infants and adults.免疫球蛋白M免疫测定法诊断婴幼儿和成人衣原体感染的准确性。
J Clin Microbiol. 1986 Nov;24(5):731-5. doi: 10.1128/jcm.24.5.731-735.1986.
7
8
Detection of chlamydial inclusions in cell culture or biopsy tissue by alkaline phosphatase-anti-alkaline phosphatase staining.通过碱性磷酸酶-抗碱性磷酸酶染色检测细胞培养物或活检组织中的衣原体包涵体。
J Clin Microbiol. 1987 Oct;25(10):1864-7. doi: 10.1128/jcm.25.10.1864-1867.1987.
9
Premarket evaluation of Monofluor reagent for detecting Chlamydia trachomatis in adolescent outpatients.用于检测青少年门诊患者沙眼衣原体的单氟试剂上市前评估。
Genitourin Med. 1988 Jun;64(3):165-8. doi: 10.1136/sti.64.3.165.
10
Comparison of buffalo green monkey cells and McCoy cells for isolation of Chlamydia trachomatis in a microtiter system.在微量滴定系统中比较水牛绿猴细胞和 McCoy 细胞用于沙眼衣原体分离的情况。
J Clin Microbiol. 1989 Oct;27(10):2364-5. doi: 10.1128/jcm.27.10.2364-2365.1989.
本文引用的文献
1
2
The rôle of Chlamydia trachomatis in genital-tract and associated diseases.沙眼衣原体在生殖道及相关疾病中的作用。
J Clin Pathol. 1980 Mar;33(3):205-33. doi: 10.1136/jcp.33.3.205.
3
Immunotypes of Chlamydia trachomatis isolates in Seattle, Washington.华盛顿州西雅图市沙眼衣原体分离株的免疫型别
Infect Immun. 1983 Aug;41(2):865-8. doi: 10.1128/iai.41.2.865-868.1983.
4
Detection of Chlamydia trachomatis inclusions in Mccoy cell cultures with fluorescein-conjugated monoclonal antibodies.使用荧光素偶联单克隆抗体检测 McCoy 细胞培养物中的沙眼衣原体包涵体。
J Clin Microbiol. 1983 Apr;17(4):666-8. doi: 10.1128/jcm.17.4.666-668.1983.
5
Sensitivity of immunofluorescence with monoclonal antibodies for detection of Chlamydia trachomatis inclusions in cell culture.用单克隆抗体进行免疫荧光检测细胞培养中沙眼衣原体包涵体的敏感性。
J Clin Microbiol. 1982 Jul;16(1):4-7. doi: 10.1128/jcm.16.1.4-7.1982.
6
Micro cell culture method for isolation of Chlamydia trachomatis.用于分离沙眼衣原体的微量细胞培养方法。
Appl Microbiol. 1974 Oct;28(4):727-9. doi: 10.1128/am.28.4.727-729.1974.
7
Detection of Chlamydia (Bedsonia) in certain infections of man. I. Laboratory procedures: comparison of yolk sac and cell culture for detection and isolation.人体某些感染中衣原体（贝宗体）的检测。一、实验室方法：用于检测和分离的卵黄囊法与细胞培养法的比较
J Infect Dis. 1969 Oct;120(4):451-62. doi: 10.1093/infdis/120.4.451.
8
Simplified microimmunofluorescence test with trachoma-lymphogranuloma venereum (Chlamydia trachomatis) antigens for use as a screening test for antibody.采用沙眼 - 性病性淋巴肉芽肿（沙眼衣原体）抗原的简易微量免疫荧光试验，用作抗体筛查试验。
J Clin Microbiol. 1975 Mar;1(3):250-5. doi: 10.1128/jcm.1.3.250-255.1975.
9
Comparative recoveries of chlamydia from urethral specimens using glass vials and plastic microtiter plates.使用玻璃瓶和塑料微量滴定板从尿道标本中衣原体的比较回收率。
Am J Clin Pathol. 1977 May;67(5):496-8. doi: 10.1093/ajcp/67.5.496.
10
Infection with Chlamydia trachomatis: involvement of multiple anatomic sites in neonates.沙眼衣原体感染：新生儿多个解剖部位受累
J Infect Dis. 1979 Feb;139(2):232-4. doi: 10.1093/infdis/139.2.232.
Zotero 插件