用单克隆抗体对微量培养板中沙眼衣原体包涵体进行间接免疫荧光染色。
Indirect immunofluorescence staining of Chlamydia trachomatis inclusions in microculture plates with monoclonal antibodies.
作者信息
Zapata M, Chernesky M, Mahony J
出版信息
J Clin Microbiol. 1984 Jun;19(6):937-9. doi: 10.1128/jcm.19.6.937-939.1984.
Abstract
Indirect immunofluorescence (IF) staining, using a monoclonal antibody, detected two- to fourfold more inclusions than did iodine staining. Of 274 clinical specimens, 53 (19.3%) were positive by IF on passage 1 as compared with 33 (12%) by iodine staining (P less than 0.005). IF-stained inclusions in McCoy cells in the bottom of microculture wells were readily viewed with a long-focal-length objective at a magnification of 250 X.
摘要
使用单克隆抗体的间接免疫荧光（IF）染色检测到的包涵体比碘染色多两到四倍。在274份临床标本中，第1代时IF检测呈阳性的有53份（19.3%），而碘染色为33份（12%）（P小于0.005）。在微量培养孔底部的 McCoy 细胞中经IF染色的包涵体，用长焦距物镜在250倍放大倍数下很容易观察到。
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