Vikha I V, Smirnova V G, Orlina E A, Osin N S, Khramov E A
Klin Lab Diagn. 1997 Apr(4):24, 33-6.
A diagnostic enzyme immunoassay test kit is proposed for detecting anti-treponema antibodies in sera of syphilis patients, usable in two variants: on prefabricated polystyrene plates or by immunofiltration using a membrane filtration enzyme immunoassay kit. A peculiar feature of the test kit is the use of enzyme immunoassay conjugate based on monoclonal antibodies to light chains of human immunoglobulins, highly purified antigen from cultural T. pallidum, two substrates for detecting enzymatic activity. The efficacy of the test was confirmed on 163 patients' and control sera and compared with that of the complement fixation test, two modifications of immunofluorescent test, and micromodification of the precipitation test. The test proved to be highly sensitive, specific, and reproducible, and is recommended as a confirmation test for the diagnosis of syphilis.
一种诊断酶免疫分析检测试剂盒被提出来用于检测梅毒患者血清中的抗梅毒螺旋体抗体，它有两种使用方式：在预制聚苯乙烯板上进行检测，或者使用膜过滤酶免疫分析试剂盒通过免疫过滤进行检测。该检测试剂盒的一个独特之处在于使用了基于抗人免疫球蛋白轻链单克隆抗体的酶免疫分析结合物、来自培养梅毒螺旋体的高度纯化抗原以及两种用于检测酶活性的底物。该检测方法在163份患者血清和对照血清上得到了验证，并与补体结合试验、两种免疫荧光试验改良方法以及沉淀试验的微量改良方法进行了比较。结果证明该检测方法具有高敏感性、特异性和可重复性，被推荐作为梅毒诊断的确证试验。
