Nakagomi T, Nakagomi O, Streckert H J
Department of Microbiology, Akita University School of Medicine, Japan.
Arch Virol. 1997;142(6):1255-62. doi: 10.1007/s007050050157.
Bovine rotavirus strain 678 is the first G8 strain of bovine origin but the literature is confusing as to its P type. In this study, two-way cross neutralization between 678 and 0510, a prototype G6P7[5] virus, was shown by plaque-reduction neutralization assays, establishing the P type of 678 as being P7[5]. The P7[5] specificity of 678 VP4 was reinforced by the finding that the VP8* portion of 678 VP4 had the highest amino acid identity with those of P7[5] bovine rotaviruses. Apparent contradiction with previous serological studies relates to intricacy of antigenicity and immunogenicity of UK VP4 in reassortants.
牛轮状病毒678株是首例源自牛的G8毒株，但关于其P型，文献记载却很混乱。在本研究中，通过蚀斑减少中和试验显示了678株与原型G6P7[5]病毒0510之间的双向交叉中和作用，确定678株的P型为P7[5]。678株VP4的P7[5]特异性因以下发现而得到加强：678株VP4的VP8部分与P7[5]牛轮状病毒的VP8部分具有最高的氨基酸同一性。与先前血清学研究的明显矛盾涉及重组体中英国VP4抗原性和免疫原性的复杂性。
