Watts D M, Lavera V, Callahan J, Rossi C, Oberste M S, Roehrig J T, Cropp C B, Karabatsos N, Smith J F, Gubler D J, Wooster M T, Nelson W M, Hayes C G
U.S. Naval Medical Research Institute Detachment, NAMRID/Unit 3800, American Embassy, Lima, Peru.
Am J Trop Med Hyg. 1997 Jun;56(6):661-7. doi: 10.4269/ajtmh.1997.56.661.
An outbreak of a febrile illness characterized by headache, ocular pain, myalgia, and arthralgia occurred during June 1994 among Peruvian army troops in Northern Peru. On June 14-16, 1994, clinical data and blood samples were obtained from eight soldiers with a febrile illness, and from 26 others who had a history of febrile illness during the past three months. A follow-up blood sample was obtained 107 days later from four of the febrile and seven of the afebrile soldiers. Serum samples were tested for dengue (DEN), Oropouche (ORO), and Venezuelan equine encephalitis (VEE) IgM and IgG antibodies by an enzyme-linked immunosorbent assay (ELISA). Virus isolation was performed by inoculation of newborn mice and Vero cell cultures. Viral isolates were identified by immunofluorescence, ELISA, and nucleotide sequencing. A VEE virus infection was confirmed in three of the eight febrile soldiers, two by virus isolation, and one by serology. Antigenic analysis indicated that one of the virus isolates was similar to VEE subtype I, variety ID, viruses previously isolated in Colombia and Venezuela. Nucleotide sequence data showed that both viral isolates were identical to one another and closely related to VEE ID viruses previously isolated in Peru, Colombia, and Venezuela. Serologic results showed that two of 26 afebrile soldiers had IgM antibody to VEE and four had IgG antibody to VEE; two febrile soldiers had IgG antibody in their first serum samples. Oropouche-specific IgM antibody was detected in one of the eight febrile and five of the afebrile soldiers, and 18 of the 34 soldiers had low titers of ORO IgG antibody titers, which did not meet the diagnostic criteria for confirmed cases. All soldiers were negative for DEN IgM antibody, and 10 had flavivirus IgG antibody that reacted with DEN antigens. These data indicated that VEE ID virus was one of the causes of illness among Peruvians soldiers and that this was the first association of this VEE subtype with human disease in Peru.
1994年6月，秘鲁北部的秘鲁军队中爆发了一种以头痛、眼痛、肌痛和关节痛为特征的发热性疾病。1994年6月14日至16日，从8名患有发热性疾病的士兵以及另外26名在过去三个月内有发热性疾病病史的士兵身上获取了临床数据和血样。107天后，从4名发热士兵和7名无发热士兵身上获取了随访血样。通过酶联免疫吸附测定（ELISA）检测血清样本中的登革热（DEN）、奥罗普切（ORO）和委内瑞拉马脑炎（VEE）IgM和IgG抗体。通过接种新生小鼠和Vero细胞培养物进行病毒分离。通过免疫荧光、ELISA和核苷酸测序对病毒分离株进行鉴定。在8名发热士兵中的3人身上确诊感染了VEE病毒，其中2人通过病毒分离确诊，1人通过血清学确诊。抗原分析表明，其中一株病毒分离株与VEE I亚型、ID变种病毒相似，此前曾在哥伦比亚和委内瑞拉分离到该病毒。核苷酸序列数据显示，两株病毒分离株彼此相同，且与此前在秘鲁、哥伦比亚和委内瑞拉分离到的VEE ID病毒密切相关。血清学结果显示，26名无发热士兵中有2人有VEE IgM抗体，4人有VEE IgG抗体；2名发热士兵的首次血清样本中有IgG抗体。在8名发热士兵中的1人以及5名无发热士兵中检测到了奥罗普切特异性IgM抗体，34名士兵中有18人奥罗IgG抗体滴度较低，未达到确诊病例的诊断标准。所有士兵的DEN IgM抗体均为阴性，10人有与DEN抗原反应的黄病毒IgG抗体。这些数据表明，VEE ID病毒是秘鲁士兵患病的原因之一，这也是该VEE亚型在秘鲁首次与人类疾病相关联。
