Aguilar Patricia V, Greene Ivorlyne P, Coffey Lark L, Medina Gladys, Moncayo Abelardo C, Anishchenko Michael, Ludwig George V, Turell Michael J, O'Guinn Monica L, Lee John, Tesh Robert B, Watts Douglas M, Russell Kevin L, Hice Christine, Yanoviak Stephen, Morrison Amy C, Klein Terry A, Dohm David J, Guzman Hilda, Travassos da Rosa Amelia P A, Guevara Carolina, Kochel Tadeusz, Olson James, Cabezas Cesar, Weaver Scott C
University of Texas Medical Branch, Galveston, Texas 77555-0609, USA.
Emerg Infect Dis. 2004 May;10(5):880-8. doi: 10.3201/eid1005.030634.
Since Venezuelan equine encephalitis virus (VEEV) was isolated in Peru in 1942, >70 isolates have been obtained from mosquitoes, humans, and sylvatic mammals primarily in the Amazon region. To investigate genetic relationships among the Peru VEEV isolates and between the Peru isolates and other VEEV strains, a fragment of the PE2 gene was amplified and analyzed by single-stranded conformation polymorphism. Representatives of seven genotypes underwent sequencing and phylogenetic analysis. The results identified four VEE complex lineages that cocirculate in the Amazon region: subtypes ID (Panama and Colombia/Venezuela genotypes), IIIC, and a new, proposed subtype IIID, which was isolated from a febrile human, mosquitoes, and spiny rats. Both ID lineages and the IIID subtype are associated with febrile human illness. Most of the subtype ID isolates belonged to the Panama genotype, but the Colombia/Venezuela genotype, which is phylogenetically related to epizootic strains, also continues to circulate in the Amazon basin.
自1942年委内瑞拉马脑炎病毒（VEEV）在秘鲁被分离出来后，已从主要位于亚马逊地区的蚊子、人类和野生哺乳动物中获得了70多个分离株。为了研究秘鲁VEEV分离株之间以及秘鲁分离株与其他VEEV毒株之间的遗传关系，对PE2基因的一个片段进行了扩增，并通过单链构象多态性进行分析。对7个基因型的代表进行了测序和系统发育分析。结果确定了在亚马逊地区共同传播的4个VEE复合谱系：ID亚型（巴拿马和哥伦比亚/委内瑞拉基因型）、IIIC型，以及一个新提出的IIID亚型，该亚型是从一名发热患者、蚊子和刺鼠中分离出来的。ID谱系和IIID亚型均与人类发热疾病有关。大多数ID亚型分离株属于巴拿马基因型，但与 epizootic 毒株在系统发育上相关的哥伦比亚/委内瑞拉基因型也继续在亚马逊盆地传播。
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