Oberste M S, Weaver S C, Watts D M, Smith J F
Virology Division, U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21702-5011, USA.
Am J Trop Med Hyg. 1998 Jan;58(1):41-6. doi: 10.4269/ajtmh.1998.58.41.
Venezuelan equine encephalitis (VEE) virus was isolated in 1993, 1994, and 1995 from human cases of acute, undifferentiated, febrile illness in the Peruvian Amazon Basin. Two virus isolates were recovered in 1994 from Peruvian soldiers at a jungle outpost near Pantoja in northern Peru, and 10 isolates were obtained from military personnel and civilians in 1993-1995 in Iquitos, an urban center in northeastern Peru. The genetic relationship of these isolates to other VEE virus strains was determined by sequencing 856-867 nucleotide reverse transcription-polymerase chain reaction fragments derived from the PE2 glycoprotein gene. The sequences were compared with those of other VEE virus strains, including representatives of the IAB, IC, ID, IE, II, and IIIC subtypes. The two Pantoja isolates were most closely related to subtype IC and ID viruses previously isolated in Colombia and Venezuela, and to the ID viruses isolated during the 1970s in Iquitos. All of the recent Iquitos isolates were similar to one another, but they were more closely related to Panamanian ID strains than to isolates previously obtained in Iquitos, Peru, or in Colombia and Venezuela. The recent Iquitos VEE viral isolates were the first Panama-genotype VEE ID virus strains identified outside of the Republic of Panama.
1993年、1994年和1995年，在秘鲁亚马逊盆地急性、未分化的发热性疾病患者中分离出委内瑞拉马脑炎（VEE）病毒。1994年，在秘鲁北部潘托哈附近丛林前哨的秘鲁士兵中分离出两株病毒，1993 - 1995年在秘鲁东北部城市中心伊基托斯的军事人员和平民中分离出10株病毒。通过对源自PE2糖蛋白基因的856 - 867个核苷酸的逆转录 - 聚合酶链反应片段进行测序，确定了这些分离株与其他VEE病毒株的遗传关系。将这些序列与其他VEE病毒株的序列进行比较，包括IAB、IC、ID、IE、II和IIIC亚型的代表株。潘托哈的两株分离株与先前在哥伦比亚和委内瑞拉分离出的IC和ID亚型病毒以及20世纪70年代在伊基托斯分离出的ID病毒关系最为密切。伊基托斯最近分离出的所有病毒株彼此相似，但它们与巴拿马的ID株的关系比与先前在秘鲁伊基托斯或哥伦比亚和委内瑞拉获得的分离株更为密切。伊基托斯最近分离出的VEE病毒株是在巴拿马共和国境外鉴定出的首批巴拿马基因型VEE ID病毒株。
