Benaron D A, Contag P R, Contag C H
Medical Free Electron Laser Program, Hansen Experimental Physics Laboratory (HEPL), Department of Physics, Stanford University, CA 94034, USA.
Philos Trans R Soc Lond B Biol Sci. 1997 Jun 29;352(1354):755-61. doi: 10.1098/rstb.1997.0059.
Light can be used to probe the function and structure of human tissues. We have been exploring two distinct methods: (i) externally emitting light into tissue and measuring the transmitted light to characterize a region through which the light has passed, and (ii) internally generating light within tissue and using the radiated light as a quantitative homing beacon. The emitted-light approach falls within the domain of spectroscopy, and has allowed for imaging of intracranial haemorrhage in newborns and of brain functions in adults. The generated-light approach is conceptually parallel to positron emission tomography (PET) or nuclear medicine scanning, and has allowed for real-time, non-invasive monitoring and imaging of infection and gene expression in vivo using low-light cameras and ordinary lenses. In this paper, we discuss recent results and speculate on the applications of such techniques.
光可用于探测人体组织的功能和结构。我们一直在探索两种不同的方法：（i）向组织外部发射光并测量透射光，以表征光通过的区域；（ii）在组织内部产生光，并将辐射光用作定量归航信标。发射光方法属于光谱学领域，已实现对新生儿颅内出血和成人脑功能的成像。产生光方法在概念上与正电子发射断层扫描（PET）或核医学扫描类似，并且已使用低光相机和普通镜头实现了对体内感染和基因表达的实时、非侵入性监测和成像。在本文中，我们讨论了近期的研究结果，并对这些技术的应用进行了推测。
