Morrey J D, Jackson M K, Bunch T D, Sidwell R W
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan 84322-5600.
Intervirology. 1993;36(2):65-71. doi: 10.1159/000150323.
Topical dinitrochlorobenzene (DNCB) is often used for evaluating contact skin hypersensitivity in immunocompromised patients. We have determined, in this study, that topical application of DNCB alone, even without induction of contact skin hypersensitivity, was sufficient to observe activation of the human immunodeficiency virus promoter (long terminal repeat) in the skin of an HIV-1 long terminal repeat-luciferase transgenic mouse model. Such treatment might be contra-indicative in patients infected with the human immunodeficiency virus, because in earlier studies DNCB-exposed skin dendritic cells might migrate into draining lymph nodes which play an important role in AIDS pathogenesis.
局部应用二硝基氯苯（DNCB）常用于评估免疫功能低下患者的接触性皮肤超敏反应。在本研究中，我们已经确定，仅局部应用DNCB，即使不诱发接触性皮肤超敏反应，也足以在HIV-1长末端重复序列-荧光素酶转基因小鼠模型的皮肤中观察到人类免疫缺陷病毒启动子（长末端重复序列）的激活。这种治疗方法可能对感染人类免疫缺陷病毒的患者有禁忌，因为在早期研究中，接触DNCB的皮肤树突状细胞可能迁移至引流淋巴结，而引流淋巴结在艾滋病发病机制中起重要作用。
