Li P, Kawachi H, Morioka T, Orikasa M, Oite T, Shi Z S, Shimizu F
Department of Cell Biology, Niigata University School of Medicine, Japan.
Pathol Int. 1997 Jul;47(7):430-5. doi: 10.1111/j.1440-1827.1997.tb04520.x.
The effects of traditional Chinese medicine (Sairei-to) on experimental glomerulonephritis induced in rats by monoclonal antibody (mAb) 1-22-3 injection was examined. The level of proteinuria in the Sairei-to-treated group was significantly lower than that in the PBS treated group. This suppressive effect was caused by the major component of Sairei-to, Syo-saiko-to but not by another component, Gorel-san. The suppressive effect of Syo-saiko-to was identified in its components (Bupleuri radix, Pinelliae tuber and Zingiberis rhizoma), but not in the other combined components (Ginseng radix and Zizyphi fructus). Further study revealed that the suppressive effects of the combined components were mainly derived from Bupleuri radix. It was demonstrated that the actual active ingredient is probably Saikosaponin-d. Light microscopy revealed that Sairei-to and its effective components suppressed the proliferation of mesangial cells and mesangial matrix expansion. Semiquantitative morphological studies of glomerular lesions on the eighth day showed that Syo-saiko-to and its combined components (Bupleuri radix, Zingiberis rhizoma and Pinelliae tuber) suppressed mesangial matrix expansion significantly compared with phosphate-buffered saline control groups (matrix score: 28.0 +/- 19.1 vs 102.3 +/- 14.1; 30.9 +/- 30.1 vs 102.3 +/- 14.1, P < 0.005, respectively). It was concluded that Saikosaponin-d, as well as Bupleuri radix, Syo-saiko-to and Sairei-to can suppress proteinuria and morphological changes in the rat glomerulonephritis model induced by mAb 1-22-3.
研究了中药（柴苓汤）对注射单克隆抗体（mAb）1-22-3诱导的大鼠实验性肾小球肾炎的影响。柴苓汤治疗组的蛋白尿水平显著低于磷酸盐缓冲液（PBS）治疗组。这种抑制作用是由柴苓汤的主要成分小柴胡汤引起的，而非另一种成分五苓散。小柴胡汤的抑制作用在其成分（柴胡、半夏和生姜）中得到证实，但在其他组合成分（人参和酸枣仁）中未得到证实。进一步研究表明，组合成分的抑制作用主要源于柴胡。结果表明，实际活性成分可能是柴胡皂苷d。光学显微镜检查显示，柴苓汤及其有效成分可抑制系膜细胞增殖和系膜基质扩张。第8天肾小球病变的半定量形态学研究表明，与磷酸盐缓冲液对照组相比，小柴胡汤及其组合成分（柴胡、生姜和半夏）可显著抑制系膜基质扩张（基质评分：分别为28.0±19.1 vs 102.3±14.1；30.9±30.1 vs 102.3±14.1，P<0.005）。得出结论，柴胡皂苷d以及柴胡、小柴胡汤和柴苓汤可抑制mAb 1-22-3诱导的大鼠肾小球肾炎模型中的蛋白尿和形态学变化。
