Kawachi H, Takashima N, Orikasa M, Oite T, Shimizu F
Department of Immunology, Niigata University, School of Medicine, Japan.
Pathol Int. 1994 May;44(5):339-44. doi: 10.1111/j.1440-1827.1994.tb02933.x.
The effects of traditional Chinese medicine (Sairei-to) on monoclonal antibody (mAb) inducing proteinuria were examined. Four hundred, 200 and 100 mg/kg body weight (BW) of Sairei-to and phosphate-buffered saline (PBS) as a control were injected intraperitoneally into four groups of female Wistar rats every day from 5 days before intravenous injection of mAb to the end of the experimental period. The amount of urinary protein excretion was significantly suppressed in roughly a dose-dependent manner. For example, 116.6 +/- 89.7 mg/day of proteinuria was observed in control groups compared to 4.2 +/- 15.2 mg/day in the 400 mg/kg BW of Sairei-to treated group 2 days after mAb injection (P < 0.01). Statistically significant (P < 0.01) differences were again observed in a repeat experiment (122.1 +/- 53.7 vs 10.2 +/- 10.1 mg/day on the 2nd day) without affecting the glomerular filtration rate. No significant difference was recognized between the total amount of mAb bound to glomeruli 1 h after mAb injection in Sairei-to-treated and non-treated rats, indicating that Sairei-to pretreatment has no effects on the number or quality of antigenic molecules. The effect of Sairei-to on a non-immunological model of proteinuria was also examined. No significant reduction of proteinuria by similar Sairei-to treatment was observed in aminonucleoside of puromycin nephropathy. The authors conclude that mAb-induced proteinuria in rats is significantly suppressed by the traditional Chinese medicine, Sairei-to.
研究了中药（柴苓汤）对单克隆抗体（mAb）诱导蛋白尿的影响。从静脉注射mAb前5天至实验期结束，每天向四组雌性Wistar大鼠腹腔注射柴苓汤，剂量分别为400、200和100mg/kg体重（BW），并以磷酸盐缓冲盐水（PBS）作为对照。尿蛋白排泄量大致呈剂量依赖性显著抑制。例如，mAb注射后2天，对照组蛋白尿为116.6±89.7mg/天，而柴苓汤400mg/kg BW治疗组为4.2±15.2mg/天（P<0.01）。在重复实验中再次观察到统计学上的显著差异（第2天分别为122.1±53.7和10.2±10.1mg/天，P<0.01），且不影响肾小球滤过率。在mAb注射后1小时，柴苓汤治疗组和未治疗组大鼠肾小球结合的mAb总量之间未发现显著差异，表明柴苓汤预处理对抗抗原分子的数量或质量没有影响。还研究了柴苓汤对非免疫性蛋白尿模型的作用。在嘌呤霉素氨基核苷肾病中，类似的柴苓汤治疗未观察到蛋白尿显著减少。作者得出结论，中药柴苓汤可显著抑制大鼠mAb诱导的蛋白尿。
