Prince A M, Whalen R, Brotman B
Laboratory of Virology and Parasitology, Lindsley F. Kimball Research Institute of the New York Blood Center, NY 10021, USA.
Vaccine. 1997 Jun;15(8):916-9. doi: 10.1016/s0264-410x(96)00248-4.
To determine whether DNA based immunization could protect newborn chimpanzees against a challenge infection with hepatitis B virus, two chimpanzees were immunized on the day of birth with a plasmid coding for hepatitis B surface antigen, and boosted at 6 and 24 weeks. Both animals produced transient antibody to the hepatitis B surface antigen. Following challenge with hepatitis B virus at 33 weeks the two immunized animals developed anamnestic antibody responses, however, neither developed detectable hepatitis B surface antigen or antibody to the core protein, the conventional markers of hepatitis B infection. Both of these markers appeared in an unimmunized control animal. We conclude that DNA based immunization can induce protective immunity in newborn chimpanzees.
为了确定基于DNA的免疫接种能否保护新生黑猩猩免受乙型肝炎病毒的攻击感染，两只黑猩猩在出生当天用编码乙型肝炎表面抗原的质粒进行免疫接种，并在6周和24周时进行加强免疫。两只动物均产生了针对乙型肝炎表面抗原的短暂抗体。在33周时用乙型肝炎病毒进行攻击后，两只免疫接种的动物产生了回忆性抗体反应，然而，两者均未出现可检测到的乙型肝炎表面抗原或核心蛋白抗体，而这些是乙型肝炎感染的传统标志物。这两种标志物均出现在一只未免疫接种的对照动物中。我们得出结论，基于DNA的免疫接种可在新生黑猩猩中诱导保护性免疫。
