Josien R, Heslan M, Soulillou J P, Cuturi M C
Institut National de la Sante et de la Recherche Medicale U437, Institut de Transplantation et de Recherche en Transplantation, Immeuble Jean Monnet, Nantes, France.
J Exp Med. 1997 Aug 4;186(3):467-72. doi: 10.1084/jem.186.3.467.
Dendritic cells (DC) are a subset of leukocytes whose major function is antigen presentation. We investigated the phenotype and function of enriched (95-98.5%) rat DC. We show that both spleen and thymus DC express the natural killer cell receptor protein 1 (NKR-P1) as a disulfide linked homodimer of 60 kD. Freshly isolated DC express a low level of NKR-P1, which is strongly upregulated after overnight culture. Spleen, but not thymus DC, were able to kill the NK-sensitive YAC-1 cell line in vitro, and since this killing was Ca2+ dependent, a Fas ligand-Fas interaction was probably not involved. Besides their potent antigen-presenting function, DC can thus be cytotoxic for some tumor targets.
树突状细胞（DC）是白细胞的一个亚群，其主要功能是抗原呈递。我们研究了富集的（95 - 98.5%）大鼠DC的表型和功能。我们发现脾脏和胸腺DC均表达自然杀伤细胞受体蛋白1（NKR-P1），它是一种60 kD的二硫键连接的同二聚体。新鲜分离的DC表达低水平的NKR-P1，过夜培养后其表达强烈上调。脾脏DC而非胸腺DC能够在体外杀伤NK敏感的YAC-1细胞系，并且由于这种杀伤依赖于Ca2+，因此可能不涉及Fas配体-Fas相互作用。因此，除了其强大的抗原呈递功能外，DC对某些肿瘤靶标也具有细胞毒性。
