Purification of the transcription/repair factor TFIIH and evaluation of its associated activities in vitro.
作者信息
Marinoni J C, Rossignol M, Egly J M
机构信息
Institut de Biologie et Génétique Moléculaire et Cellulaire, UPR 6520 (CNRS), Unité 184 (INSERM), Illkirch, France.
出版信息
Methods. 1997 Jul;12(3):235-53. doi: 10.1006/meth.1997.0476.
PMID:9237168
Abstract
We describe here the methodology developed in our laboratory to study the role of TFIIH, a multisubunit protein complex, in the various mechanisms of cell life: transcription, DNA repair, and cell cycle regulation. Protocols are given to purify TFIIH and to study its various enzymatic activities as well as its transcription and nucleotide excision repair activities.
摘要
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