Maeda K, Yokoyama N, Fujita K, Mikami T
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Virus Genes. 1997;14(2):105-9. doi: 10.1023/a:1007961100023.
The feline herpesvirus type 1 (FHV-1) gene encoding glycoprotein C (gC) has been sequenced and identified based on its genomic location and comparative analysis to other alphaherpesvirus gCs, and the expressed gC protein was also identified by using specific monoclonal antibodies. The FHV-1 gC gene was located within a 7.0 kbp EcoRI fragment, and was 1602 bp in length. The amino acid sequence deduced from the nucleotide sequence was predicted to encode a membrane glycoprotein containing a characteristic N-terminal hydrophobic signal sequence, nine potential N-linked glycosylation sites, and C-terminal transmembrane and cytoplasmic domains. The FHV-1 gC was expressed in COS-7 cells. When flowcytometric analysis was carried out, the gC expressed in COS-7 cells reacted with a panel of monoclonal antibodies against gp113: By immunoprecipitation analysis, the gC expressed in COS-7 cells possessed molecular masses of 125-150 kilodalton, and was similar in size to that in FHV-1-infected CRFK cells.
编码糖蛋白C（gC）的猫疱疹病毒1型（FHV-1）基因已根据其基因组位置进行测序和鉴定，并与其他α疱疹病毒的gC进行了比较分析，同时还使用特异性单克隆抗体鉴定了表达的gC蛋白。FHV-1 gC基因位于一个7.0 kbp的EcoRI片段内，长度为1602 bp。从核苷酸序列推导的氨基酸序列预计编码一种膜糖蛋白，该蛋白含有一个特征性的N端疏水信号序列、九个潜在的N-糖基化位点以及C端跨膜和胞质结构域。FHV-1 gC在COS-7细胞中表达。进行流式细胞术分析时，COS-7细胞中表达的gC与一组抗gp113的单克隆抗体发生反应：通过免疫沉淀分析，COS-7细胞中表达的gC的分子量为125 - 150千道尔顿，大小与FHV-1感染的CRFK细胞中的相似。
