Burke W J, Schmitt C A, Miller C, Li S W
Department of Neurology, Saint Louis University Medical School and Veterans Affairs Medical Center, MO 63110, USA.
Brain Res. 1997 Jun 20;760(1-2):290-3. doi: 10.1016/s0006-8993(97)00447-2.
3,4-Dihydroxyphenylglycolaldehyde (DOPEGAL) is the monoamine oxidase A metabolite of norepinephrine and epinephrine. DOPEGAL, but not other metabolites, kills differentiated PC-12 cells. However, the type of DOPEGAL induced cell death, whether necrosis or apoptosis, is not known. To determine the type of cell death triggered by DOPEGAL, PC-12 cells cultured in the presence or absence of 30 microM DOPEGAL were examined by electron microscopy and DNA agarose gel electrophoresis for characteristic features of apoptosis. Results indicate that DOPEGAL induces apoptosis in these cells. Implications for degenerative diseases are discussed.
3,4-二羟基苯乙醇醛（DOPEGAL）是去甲肾上腺素和肾上腺素的单胺氧化酶A代谢产物。DOPEGAL而非其他代谢产物可杀死分化的PC-12细胞。然而，DOPEGAL诱导的细胞死亡类型，无论是坏死还是凋亡，尚不清楚。为了确定DOPEGAL引发的细胞死亡类型，通过电子显微镜和DNA琼脂糖凝胶电泳检查了在存在或不存在30微摩尔DOPEGAL的情况下培养的PC-12细胞，以寻找凋亡的特征。结果表明DOPEGAL在这些细胞中诱导凋亡。文中讨论了其对退行性疾病的影响。
