Henderson A J, Calame K L
Department of Microbiology, Columbia University, College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032, USA.
Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8714-9. doi: 10.1073/pnas.94.16.8714.
The importance of CCAAT/enhancer binding proteins (C/EBPs) and binding sites for HIV-1 replication in primary macrophages, T cell lines and primary CD4(+) T cells was examined. When lines overexpressing the C/EBP dominant-negative protein LIP were infected with HIV-1, replication occurred in Jurkat T cells but not in U937 promonocytes, demonstrating a requirement for C/EBP activators by HIV-1 only in promonocytes. Primary macrophages did not support the replication of HIV-1 harboring mutant C/EBP binding sites in the long terminal repeat but Jurkat, H9 and primary CD4(+) T cells supported replication of wild-type and mutant HIV-1 equally well. Thus the requirement for C/EBP sites is also confined to monocyte/macrophages. The requirement for C/EBP proteins and sites identifies the first uniquely macrophage-specific regulatory mechanism for HIV-1 replication.
研究了CCAAT/增强子结合蛋白（C/EBP）及结合位点在原代巨噬细胞、T细胞系和原代CD4(+) T细胞中对HIV-1复制的重要性。当用HIV-1感染过表达C/EBP显性负性蛋白LIP的细胞系时，HIV-1在Jurkat T细胞中发生复制，但在U937原单核细胞中不发生复制，这表明HIV-1仅在原单核细胞中需要C/EBP激活剂。原代巨噬细胞不支持在长末端重复序列中含有突变C/EBP结合位点的HIV-1的复制，但Jurkat、H9和原代CD4(+) T细胞对野生型和突变型HIV-1的复制支持程度相同。因此，对C/EBP位点的需求也仅限于单核细胞/巨噬细胞。对C/EBP蛋白和位点的需求确定了HIV-1复制首个独特的巨噬细胞特异性调节机制。
