华支睾吸虫病的免疫印迹模式。

Immunoblot patterns of clonorchiasis.

作者信息

Hong S T, Kho W G, Lee M, Lee J S, Lee S H

机构信息

Department of Parasitology, Seoul National University College of Medicine, Korea.

出版信息

Korean J Parasitol. 1997 Jun;35(2):87-93. doi: 10.3347/kjp.1997.35.2.87.

DOI:10.3347/kjp.1997.35.2.87

PMID:9241982

Abstract

Clonorchis sinensis is a liver fluke which is the most prevalent helminth of humans in Korea. The better diagnostic measure of clonorchiasis is required for its nationwide control program. The present study observed antigenic bands of C. sinensis and reacting immunoglobulins in serum of infected residents. Adult C. sinensis were recovered from experimentally infected rabbits and soluble crude extract of the worms was used as the antigen after supplementation of E-64, a cysteine proteinase inhibitor. SDS-PAGE of the crude antigen resolved more than 20 protein bands between 200 and 14 kDa. The sera of infected humans collected at an endemic village showed specific IgG and IgE antibodies but little IgM and IgA antibodies. The protein bands of 94, 80, 72, 68, 52, 47, 43, 37, 34, and 28-25 kDa strongly reacted with serum Ig(GMA) or IgG antibody and 64, 62, 52, 47, 44, 34, 28, and 26 kDa bands reacted with serum IgE antibody. However, the 94, 80, 72, 68, 64, 62, 52, 47, and 40 kDa bands of C. sinensis antigen were found non-specific. The protein bands of 43, 34, and 28-25 kDa of C. sinesis are primary target molecules of further analysis.

摘要

华支睾吸虫是一种肝吸虫，是韩国人群中最常见的蠕虫。为了实施全国性的华支睾吸虫病防控项目，需要更好的诊断方法。本研究观察了华支睾吸虫的抗原条带以及感染居民血清中的反应性免疫球蛋白。从实验感染的兔子体内获取成虫华支睾吸虫，将虫体的可溶性粗提物补充半胱氨酸蛋白酶抑制剂E-64后用作抗原。粗抗原的SDS-PAGE分析在200至14 kDa之间分辨出20多条蛋白条带。在一个流行村采集的感染人群血清显示出特异性IgG和IgE抗体，但IgM和IgA抗体很少。94、80、72、68、52、47、43、37、34以及28 - 25 kDa的蛋白条带与血清Ig(GMA)或IgG抗体强烈反应，64、62、52、47、44、34、28以及26 kDa的条带与血清IgE抗体反应。然而，发现华支睾吸虫抗原的94、80、72、68、64、62、52、47以及40 kDa条带是非特异性的。华支睾吸虫43、34以及28 - 25 kDa的蛋白条带是进一步分析的主要靶分子。