从盘基网柄菌核糖体蛋白和RNA重建功能性真核核糖体。

Reconstitution of functional eukaryotic ribosomes from Dictyostelium discoideum ribosomal proteins and RNA.

作者信息

Mangiarotti G, Chiaberge S

机构信息

Department of Clinical and Biological Sciences, University of Turin, Ospedale San Luigi Gonzaga, Regione Gonzole 10, 10043 Orbassano-Torino, Italy.

出版信息

J Biol Chem. 1997 Aug 8;272(32):19682-7. doi: 10.1074/jbc.272.32.19682.

DOI:10.1074/jbc.272.32.19682

PMID:9242623

Abstract

40 and 60 S ribosomal subunits have been reconstituted in vitro from purified ribosomal RNA and ribosomal proteins of Dictyostelium discoideum. The functionality of the reconstituted ribosomes was demonstrated in in vitro mRNA-directed protein synthesis. The reassembly proceeded well with immature precursors of ribosomal RNA but poorly if at all with mature cytoplasmic RNA species. Reassembly also required a preparation of small nuclear RNA(s), acting as morphopoietic factor(s).

摘要

已从盘基网柄菌纯化的核糖体RNA和核糖体蛋白在体外重建了40S和60S核糖体亚基。重建核糖体的功能在体外mRNA指导的蛋白质合成中得到了证明。核糖体RNA的未成熟前体进行重建效果良好，但如果是成熟的细胞质RNA种类，重建效果则很差，甚至根本无法进行重建。重建还需要制备小核RNA，其作为形态发生因子发挥作用。