Ohnishi T, Watanabe S
Department of Dermatology, Teikyo University School of Medicine, Tokyo, Japan.
J Cutan Pathol. 1997 Jul;24(6):370-6. doi: 10.1111/j.1600-0560.1997.tb00806.x.
Immunophenotypes, especially expression of cytokeratins, in 9 cases of clear cell syringoma were examined using antibodies against epithelial membrane antigen (EMA), and 17 kinds of monoclonal anti-keratin antibodies to investigate its histogenesis. In addition, 7 cases of conventional syringoma were selected for parallel assessment. Conventional syringoma expressed CK1 and CK10, which exists in the acrosyringium and the transitional portion between the acrosyringium and the dermal duct. Based on immunostaining with RCK102 and 35 beta H11, syringoma was thought to express CK5. Because expression of CK5 was observed in the basal cells of sweat duct ridge (lower acrosyringium) and the outer cells of the dermal duct, but not in the acrosyringium located at upper epidermis, we speculated that syringoma differentiated toward the transitional portion between the acrosyringium and the dermal duct. A comparative study of keratin expression between conventional and clear cell syringoma showed that there was no difference in the immunoreactivities. Based on the above observations, we confirmed that clear cell syringoma is a metabolic variant of conventional syringoma, and differentiates into the transitional portion between the acrosyringium and the dermal duct.
利用抗上皮膜抗原（EMA）抗体及17种单克隆抗角蛋白抗体，检测9例透明细胞汗管瘤的免疫表型，尤其是细胞角蛋白的表达，以研究其组织发生。此外，选取7例传统汗管瘤进行平行评估。传统汗管瘤表达存在于汗腺末端及汗腺末端与真皮导管过渡部位的CK1和CK10。基于RCK102和35βH11免疫染色，认为汗管瘤表达CK5。因为在汗腺导管嵴（汗腺末端下部）的基底细胞及真皮导管的外层细胞中观察到CK5表达，而位于表皮上部的汗腺末端未观察到，所以推测汗管瘤向汗腺末端与真皮导管的过渡部位分化。传统汗管瘤与透明细胞汗管瘤角蛋白表达的比较研究显示，免疫反应性无差异。基于上述观察结果，我们证实透明细胞汗管瘤是传统汗管瘤的一种代谢变异型，并向汗腺末端与真皮导管的过渡部位分化。
