由不同群体的COPI被膜小泡进行的双向运输。

Bidirectional transport by distinct populations of COPI-coated vesicles.

作者信息

Orci L, Stamnes M, Ravazzola M, Amherdt M, Perrelet A, Söllner T H, Rothman J E

机构信息

Department of Morphology, Faculty of Medicine, University of Geneva Medical Center, Switzerland.

出版信息

Cell. 1997 Jul 25;90(2):335-49. doi: 10.1016/s0092-8674(00)80341-4.

DOI:10.1016/s0092-8674(00)80341-4

PMID:9244307

Abstract

Electron microscope immunocytochemistry reveals that both anterograde-directed (proinsulin and VSV G protein) and retrograde-directed (the KDEL receptor) cargo are present in COPI-coated vesicles budding from every level of the Golgi stack in whole cells; however, they comprise two distinct populations that together can account for at least 80% of the vesicles budding from Golgi cisternae. Segregation of anterograde- from retrograde-directed cargo into distinct sets of COPI-coated vesicles is faithfully reproduced in the cell-free Golgi transport system, in which VSV G protein and KDEL receptor are packaged into separable vesicles, even when budding is driven by highly purified coatomer and a recombinant ARF protein.

摘要

电子显微镜免疫细胞化学显示，在全细胞中，从高尔基体堆栈各层芽生出的COPI被膜小泡中同时存在顺向运输的货物（胰岛素原和VSV G蛋白）和逆向运输的货物（KDEL受体）；然而，它们构成两个不同的群体，共同至少占从高尔基池芽生出的小泡的80%。在无细胞高尔基体运输系统中，顺向运输与逆向运输的货物被分隔到不同的COPI被膜小泡中，这一过程得到了如实再现，在该系统中，即使出芽由高度纯化的外被体蛋白和重组ARF蛋白驱动，VSV G蛋白和KDEL受体也被包装到可分离的小泡中。