盘基网柄菌中肌醇磷酸代谢的生物化学与遗传学

Biochemistry and genetics of inositol phosphate metabolism in Dictyostelium.

作者信息

van Haastert P J, van Dijken P

机构信息

Department of Biochemistry, University of Groningen, The Netherlands.

出版信息

FEBS Lett. 1997 Jun 23;410(1):39-43. doi: 10.1016/s0014-5793(97)00415-8.

DOI:10.1016/s0014-5793(97)00415-8

PMID:9247119

Abstract

Biochemical and genetic data on the metabolism of inositol phosphates in the microorganism Dictyostelium are combined in a scheme composed of in five subroutes. The first subroute is the inositol cycle as found in other organisms: inositol is incorporated into phospholipids that are hydrolysed by PLC producing Ins(1,4,5)P3 which is dephosphorylated to inositol. The second subroute is the sequential phosphorylation of inositol to InsP6; the Ins(3,4,6)P3 intermediate does not release Ca2+. The third subroute is the sequential phosphorylation of Ins(1,4,5)P3 to InsP6 in a nucleus associated fraction, whereas the fourth subroute is the dephosphorylation of Ins(1,3,4,5,6)P5 to Ins(1,4,5)P3 at the plasma membrane. This last route mediates Ins(1,4,5)P3 formation in cells with a disruption of the single PLC gene. Finally, we recognize the formation of InsP7 and InsP8 as the fifth subroute.

摘要

关于黏菌中肌醇磷酸代谢的生化和遗传数据，被整合到一个由五个子途径组成的方案中。第一个子途径是在其他生物体中发现的肌醇循环：肌醇被整合到磷脂中，磷脂被磷脂酶C水解产生肌醇-1,4,5-三磷酸（Ins(1,4,5)P3），后者被去磷酸化成为肌醇。第二个子途径是肌醇依次磷酸化生成肌醇六磷酸（InsP6）；中间产物肌醇-3,4,6-三磷酸（Ins(3,4,6)P3）不会释放钙离子。第三个子途径是在细胞核相关组分中，肌醇-1,4,5-三磷酸（Ins(1,4,5)P3）依次磷酸化生成肌醇六磷酸（InsP6），而第四个子途径是在质膜上，肌醇-1,3,4,5,6-五磷酸（Ins(1,3,4,5,6)P5）去磷酸化成为肌醇-1,4,5-三磷酸（Ins(1,4,5)P3）。最后这条途径在单个磷脂酶C基因被破坏的细胞中介导肌醇-1,4,5-三磷酸（Ins(1,4,5)P3）的形成。最后，我们将肌醇七磷酸（InsP7）和肌醇八磷酸（InsP8）的形成视为第五个子途径。