Riechmann V, Irion U, Wilson R, Grosskortenhaus R, Leptin M
Institut für Genetik, Universität zu Köln, Germany.
Development. 1997 Aug;124(15):2915-22. doi: 10.1242/dev.124.15.2915.
The primordia for heart, fat body, and visceral and somatic muscles arise in specific areas of each segment in the Drosophila mesoderm. We show that the primordium of the somatic muscles, which expresses high levels of twist, a crucial factor of somatic muscle determination, is lost in sloppy-paired mutants. Simultaneously, the primordium of the visceral muscles is expanded. The visceral muscle and fat body primordia require even-skipped for their development and the mesoderm is thought to be unsegmented in even-skipped mutants. However, we find that even-skipped mutants retain the segmental modulation of the expression of twist. Both the domain of even-skipped function and the level of twist expression are regulated by sloppy-paired. sloppy-paired thus controls segmental allocation of mesodermal cells to different fates.
在果蝇中胚层的每个节段的特定区域，心脏、脂肪体以及内脏和体壁肌肉的原基会出现。我们发现，体壁肌肉的原基表达高水平的twist，这是体壁肌肉决定的关键因子，而在sloppy-paired突变体中该原基会缺失。同时，内脏肌肉的原基会扩张。内脏肌肉和脂肪体原基的发育需要even-skipped，并且在even-skipped突变体中，中胚层被认为是不分节的。然而，我们发现even-skipped突变体保留了twist表达的节段性调控。even-skipped的功能域和twist的表达水平都受sloppy-paired调控。因此，sloppy-paired控制中胚层细胞向不同命运的节段性分配。
