Ishikawa N, Wakelin D, Mahida Y R
Division of Gastroenterology, University of Nottingham, England.
Gastroenterology. 1997 Aug;113(2):542-9. doi: 10.1053/gast.1997.v113.pm9247474.
BACKGROUND & AIMS: The four principal types of intestinal epithelial cells are derived from multipotent stem cells. Currently, there is no information on factors that regulate commitment of stem cells to differentiate along one lineage vs. another. The aim of our study was to investigate the role of T cells in the regulation of small intestinal goblet cell hyperplasia in mice infected with the parasite Trichinella spiralis.
NIH mice were infected with T. spiralis, and intestinal goblet cells and cytokine response were studied. Interferon gamma and interleukin 5 were used as candidate T helper (Th)1 and Th2 cytokines, respectively. Adoptive transfer experiments were also performed.
Small intestinal goblet cell hyperplasia occurred 8 days after infection with T. spiralis. Th1-type cells were predominant in the mesenteric lymph nodes early in the course of infection, with a switch to Th2-predominant cells around the time of goblet cell hyperplasia. Transfer of Th2-enriched mesenteric lymph node cells further enhanced goblet cell hyperplasia in recipient mice. Neutralization of interleukin 5 activity did not affect T. spiralis-induced goblet cell hyperplasia.
Small intestinal goblet cell hyperplasia in T. spiralis-infected mice is probably regulated by Th2 cells. We postulate that Th2-derived factors (other than interleukin 5) induce stem cells to differentiate preferentially along the goblet cell lineage.
四种主要类型的肠上皮细胞源自多能干细胞。目前，尚无关于调节干细胞沿一种谱系而非另一种谱系分化的因素的信息。我们研究的目的是调查T细胞在感染旋毛虫的小鼠小肠杯状细胞增生调节中的作用。
用旋毛虫感染NIH小鼠，并研究肠道杯状细胞和细胞因子反应。分别使用干扰素γ和白细胞介素5作为候选辅助性T（Th）1和Th2细胞因子。还进行了过继转移实验。
感染旋毛虫8天后出现小肠杯状细胞增生。在感染过程早期，肠系膜淋巴结中Th1型细胞占主导，在杯状细胞增生时转变为以Th2型细胞为主。富含Th2的肠系膜淋巴结细胞的转移进一步增强了受体小鼠的杯状细胞增生。白细胞介素5活性的中和不影响旋毛虫诱导的杯状细胞增生。
旋毛虫感染小鼠的小肠杯状细胞增生可能受Th2细胞调节。我们推测Th2衍生因子（白细胞介素5除外）诱导干细胞优先沿杯状细胞谱系分化。
