Redhead C, Sullivan S K, Koseki C, Fujiwara K, Edwards J C
Max Planck Institut fur Zuchtungforschung, Köln, Germany.
Mol Biol Cell. 1997 Apr;8(4):691-704. doi: 10.1091/mbc.8.4.691.
p64 is an intracellular chloride channel originally identified in bovine kidney microsomes. Using a combination of immunofluorescent and electron microscopic technique, we demonstrate that p64 resides in the limiting membranes of perinuclear dense core vesicles which appear to be regulated secretory vesicles. Heterologous expression of p64 in PancI cells, a cell type which does not normally express p64, results in targeting to a similar compartment. Mutagenesis experiments demonstrate that both the N- and C-terminal domains of the protein independently contribute to subcellular distribution of the protein. The C-terminal domain functions to prevent expression of p64 on the plasma membrane and the N-terminal domain is necessary to deliver p64 to the appropriate membrane compartment.
p64是一种最初在牛肾微粒体中发现的细胞内氯离子通道。通过结合免疫荧光和电子显微镜技术，我们证明p64存在于核周致密核心囊泡的界膜中，这些囊泡似乎是受调控的分泌囊泡。p64在通常不表达p64的PancI细胞中的异源表达导致其靶向类似的区室。诱变实验表明，该蛋白的N端和C端结构域独立地对蛋白的亚细胞分布起作用。C端结构域的功能是防止p64在质膜上表达，而N端结构域是将p64递送至适当膜区室所必需的。
