Stallknecht D E, Howerth E W, Kellogg M L, Quist C F, Pisell T
College of Veterinary Medicine, University of Georgia, Athens 30602, USA.
J Wildl Dis. 1997 Jul;33(3):574-83. doi: 10.7589/0090-3558-33.3.574.
In vitro and in vivo infections were conducted to determine if the epizootic hemorrhagic disease (EHD) and bluetongue (BT) viruses would replicate in peripheral blood mononuclear (PBM) cells of white-tailed deer (Odocoileus virginianus). All of the North American EHD and BT viruses (EHD virus serotypes 1 and 2, and BT virus serotypes 2, 10, 11, 13, and 17) replicated in vitro in cultures of white-tailed deer PBM cells. However, this replication appeared to be monocyte-dependent and was not enhanced by lymphocyte blastogenesis induced by the addition of concanavalin A. In white-tailed deer infected with either EHD virus serotype 2 or BT virus serotype 10, virus could be isolated consistently from PBM cells only from post-infection day 4 through 8, although they remained viremic through post-infection day 21. In deer, highest viral titers were associated with the erythrocyte fraction, and in no cases did viral titers detected in the platelet, PBM cell or polymorphonuclear cell fractions approach titers observed in whole blood. In the in vitro infections of white-tailed deer erythrocytes, the EHD and BT viruses were associated with pits in the erythrocyte membrane. This association may be important in the long-term viremia observed in deer.
进行了体外和体内感染实验，以确定 epizootic hemorrhagic disease（EHD）病毒和蓝舌病（BT）病毒是否会在白尾鹿（Odocoileus virginianus）的外周血单核细胞（PBM）中复制。所有北美 EHD 和 BT 病毒（EHD 病毒血清型 1 和 2，以及 BT 病毒血清型 2、10、11、13 和 17）都能在体外白尾鹿 PBM 细胞培养物中复制。然而，这种复制似乎依赖于单核细胞，并且不会因添加伴刀豆球蛋白 A 诱导的淋巴细胞增殖而增强。在感染 EHD 病毒血清型 2 或 BT 病毒血清型 10 的白尾鹿中，仅在感染后第 4 天至第 8 天能持续从 PBM 细胞中分离出病毒，尽管它们在感染后第 21 天仍处于病毒血症状态。在鹿中，最高病毒滴度与红细胞部分相关，在任何情况下，血小板、PBM 细胞或多形核细胞部分中检测到的病毒滴度都未接近全血中观察到的滴度。在体外感染白尾鹿红细胞时，EHD 和 BT 病毒与红细胞膜上的凹坑有关。这种关联可能对在鹿中观察到的长期病毒血症很重要。
