Scott D A, Coulter W A, Biagioni P A, O'Neill H O, Lamey P J
School of Clinical Dentistry, Queen's University of Belfast, Northern Ireland.
J Oral Pathol Med. 1997 Aug;26(7):305-9. doi: 10.1111/j.1600-0714.1997.tb00220.x.
Recrudescent herpes labialis (RHL) is a disease caused by herpes simplex virus (HSV), predominantly type 1 (HSV-1). We have monitored HSV-1 shedding in the oral cavity by polymerase chain reaction (PCR) and enzyme-linked immunosorbent assays (ELISA) using digoxigenin-labeled primers designed to amplify a 278 bp segment of the HSV-1 UL 42 region. Prodromal RHL was confirmed by thermographic imaging in 22 patients. Infectious virus was not detected using tissue culture for virus isolation (0/22). Using PCR and agarose gel electrophoresis, we could detect HSV-1 DNA in 8/22 patients. Using a biotinylated-probe internal to the predicted sequence of the PCR product, HSV-1 DNA was detected in 10/22 patients by ELISA. We conclude that HSV-1 DNA is shed into the oral cavity of patients presenting with sub-clinical RHL and that the PCR-ELISA technique represents a more sensitive method to monitor HSV-1 shedding than conventional tissue culturing or PCR-electrophoresis alone.
复发性唇疱疹（RHL）是一种由单纯疱疹病毒（HSV）引起的疾病，主要是1型单纯疱疹病毒（HSV-1）。我们使用地高辛标记的引物通过聚合酶链反应（PCR）和酶联免疫吸附测定（ELISA）监测口腔中HSV-1的脱落情况，这些引物旨在扩增HSV-1 UL 42区域的278 bp片段。通过热成像在22例患者中确诊前驱性RHL。使用组织培养进行病毒分离未检测到感染性病毒（0/22）。通过PCR和琼脂糖凝胶电泳，我们在8/22例患者中检测到HSV-1 DNA。使用位于PCR产物预测序列内部的生物素化探针，通过ELISA在10/22例患者中检测到HSV-1 DNA。我们得出结论，HSV-1 DNA会脱落到出现亚临床RHL的患者口腔中，并且PCR-ELISA技术是一种比传统组织培养或单独的PCR-电泳更敏感的监测HSV-1脱落的方法。
