利用随机扩增多态性DNA衍生的PCR引物对益生菌鼠李糖乳杆菌进行菌株特异性鉴定。
Strain-specific identification of probiotic Lactobacillus rhamnosus with randomly amplified polymorphic DNA-derived PCR primers.
作者信息
Tilsala-Timisjärvi A, Alatossava T
机构信息
Department of Biology, University of Oulu, Linnanmaa, FIN-90570 Oulu, Finland.
出版信息
Appl Environ Microbiol. 1998 Dec;64(12):4816-9. doi: 10.1128/AEM.64.12.4816-4819.1998.
Abstract
In the present work, strain-specific PCR primers for Lactobacillus rhamnosus Lc 1/3 are described. The randomly amplified polymorphic DNA (RAPD) technique was used to produce potential strain-specific markers. They were screened for specificity by hybridization with DNA from 11 L. rhamnosus strains. A 613-bp RAPD marker found to be strain-specific was sequenced, and a primer pair specific to L. rhamnosus Lc 1/3 was constructed based on the sequence. The primer pair was tested with 11 Lactobacillus species and 11 L. rhamnosus strains and was found to be strain specific. The nucleotide sequence of the specific RAPD marker was found to contain part of a protein encoding region which showed significant similarity to several transposases for insertion sequence elements of various bacteria, including other lactic acid bacterium species.
摘要
在本研究中，描述了用于鼠李糖乳杆菌Lc 1/3的菌株特异性PCR引物。采用随机扩增多态性DNA（RAPD）技术来产生潜在的菌株特异性标记。通过与11株鼠李糖乳杆菌的DNA杂交来筛选它们的特异性。对一个被发现具有菌株特异性的613 bp RAPD标记进行测序，并基于该序列构建了对鼠李糖乳杆菌Lc 1/3特异的引物对。用11种乳杆菌属细菌和11株鼠李糖乳杆菌对该引物对进行测试，发现其具有菌株特异性。发现该特异性RAPD标记的核苷酸序列包含一个蛋白质编码区域的一部分，该区域与包括其他乳酸菌物种在内的各种细菌的插入序列元件的几种转座酶具有显著相似性。
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