Viveros Robert D, Liberman Alexander, Trogler William C, Kummel Andrew C
Department of Nanoengineering, University of California , San Diego, 4112 Pacific Hall, 9500 Gilman Drive 0358, La Jolla, California 92093.
Materials Science and Engineering Program, University of California , San Diego, 4112 Pacific Hall, 9500 Gilman Drive 0358, La Jolla, California 92093.
J Vac Sci Technol B Nanotechnol Microelectron. 2015 May;33(3):031803. doi: 10.1116/1.4916627. Epub 2015 Apr 1.
A simple method for trace elemental determination in biological tissue has been developed. Novel nanomaterials with biomedical applications necessitate the determination of the fate of the materials to understand their toxicological profile. Hollow iron-doped calcined silica nanoshells have been used as a model to demonstrate that potassium hydroxide and bath sonication at 50 °C can extract elements from alkaline-soluble nanomaterials. After alkali digestion, nitric acid is used to adjust the H into a suitable range for analysis using techniques such as inductively coupled plasma optical emission spectrometry which require neutral or acidic analytes. In chicken liver phantoms injected with the nanoshells, 96% of the expected silicon concentration was detected. This value was in good agreement with the 94% detection efficiency of nanoshells dissolved in aqueous solution as a control for potential sample matrix interference. Nanoshell detection was further confirmed in a mouse 24 h after intravenous administration; the measured silica above baseline was 35 times greater or more than the standard deviations of the measurements. This method provides a simple and accurate means to quantify alkaline-soluble nanomaterials in biological tissue.
已开发出一种用于生物组织中痕量元素测定的简单方法。具有生物医学应用的新型纳米材料需要确定其材料的去向，以了解其毒理学特征。中空铁掺杂煅烧二氧化硅纳米壳已被用作模型，以证明氢氧化钾和50°C的浴式超声处理可以从碱溶性纳米材料中提取元素。碱消解后，使用硝酸将H调节到合适的范围，以便使用电感耦合等离子体发射光谱法等需要中性或酸性分析物的技术进行分析。在注射了纳米壳的鸡肝模型中，检测到了预期硅浓度的96%。该值与作为潜在样品基质干扰对照的溶解在水溶液中的纳米壳94%的检测效率高度一致。在静脉给药24小时后的小鼠中进一步证实了纳米壳的检测；测得的高于基线的二氧化硅比测量的标准偏差大35倍或更多。该方法提供了一种简单而准确的手段来定量生物组织中的碱溶性纳米材料。
