Sargento L, Zabala L, Gomes T, Saldanha C, Martins-Silva J, Souza-Ramalho P
Faculdade Medicina de Lisboa, Dep. Oftalmologia, Hosp. Santa Maria.
Acta Med Port. 1996 Oct-Dec;9(10-12):303-7.
Chorioretinal angiography with fluorescein is an auxiliary method widely used in Ophthalmology which enables us to study the blood/humour and blood/retina/choroid interface. The presence of a strange compound in circulation may interfere with blood flow homeostasis. With the aim of studying fluorescein influence in blood rheology, blood samples were drawn before, immediately after and 30 minutes after fluorescein bolus injection; from 10 patients with diabetes mellitus, with or without retinopathy, blood fluidity (hematocrit, erythrocyte aggregation and whole blood viscosity) and erythrocyte membrane functional properties were determined. After fluorescein injection there was erythrocyte hyperaggregation (37.7%, p = 0.004), was detected, hemoglobin concentration (p = 0.039) and hematocrit (p = 0.013) decrease, and a double time increase of methemoglobin concentration (p < 0.001) the erythrocyte membrane hydrophobic region became more rigid (p = 0.016). All these abnormalities normalized after 30 minutes. In conclusion, fluorescein angiography interferes acutely with the hemorheological parameters of patients with diabetes mellitus, with erythrocyte hyperaggregation which could interfere with the microcirculation of these patients.
荧光素脉络膜视网膜血管造影是眼科广泛使用的一种辅助方法，它使我们能够研究血液/体液以及血液/视网膜/脉络膜界面。循环中存在的一种奇怪化合物可能会干扰血流稳态。为了研究荧光素对血液流变学的影响，在荧光素团注注射前、注射后即刻以及注射后30分钟采集血样；从10例患有或未患有视网膜病变的糖尿病患者中，测定血液流动性（血细胞比容、红细胞聚集和全血粘度）以及红细胞膜功能特性。荧光素注射后出现红细胞过度聚集（37.7%，p = 0.004），检测到血红蛋白浓度（p = 0.039）和血细胞比容（p = 0.013）降低，高铁血红蛋白浓度加倍升高（p < 0.001），红细胞膜疏水区域变得更加僵硬（p = 0.016）。所有这些异常在30分钟后恢复正常。总之，荧光素血管造影会急性干扰糖尿病患者的血液流变学参数，出现红细胞过度聚集，这可能会干扰这些患者的微循环。
