Wüchner C, Hilbert K, Zabel B, Winterpacht A
Children's Hospital, University of Mainz, Germany.
Hum Genet. 1997 Aug;100(2):215-9. doi: 10.1007/s004390050493.
We have determined the nucleotide sequence of the human fibroblast growth factor receptor 3 (FGFR3) gene, including 800 bp of the 5'-flanking region and compared the sequence with the previously published murine Fgfr3 gene. The organization of the gene is highly conserved between man and mouse. We used the intron sequences to design a set of primers that allow amplification of the 17 exons (2-18) that encode the complete open reading frame. Using these primers the FGFR3 gene can be amplified at the genomic level, which significantly facilitates mutational screening.
我们已经确定了人类成纤维细胞生长因子受体3（FGFR3）基因的核苷酸序列，包括800 bp的5'侧翼区域，并将该序列与先前发表的小鼠Fgfr3基因进行了比较。该基因的结构在人和小鼠之间高度保守。我们利用内含子序列设计了一组引物，可用于扩增编码完整开放阅读框的17个外显子（2 - 18）。使用这些引物，可以在基因组水平上扩增FGFR3基因，这显著便于进行突变筛查。
