Naranjo J A, Martínez-Victoria E, Valverde A, Yago M D, Mañas M
Department of Physiology, University of Granada, Spain.
Arch Physiol Biochem. 1997 Apr;105(2):144-50. doi: 10.1076/apab.105.2.144.12930.
The effect of age on the exocrine pancreatic function was studied in fifteen Granadina goat kids during the second, third and fourth postnatal week. The animals, chronically catheterized, were bottle-fed on goat milk twice a day. Pure pancreatic juice was collected from 1 hour before and throughout 8 hours after milk ingestion. Pancreatic flow rate, bicarbonate and chloride concentrations, total protein content and enzyme activities were determined. In resting conditions, pancreatic flow rate augmented with age, probably reflecting an increase in body and pancreas weight. Resting amylase and lipase activities recorded significant increases during the fourth week of life. That may be associated, respectively, to the immediate change to a high-starch diet and to a simultaneous decrease in the salivary lipase levels. Our results also showed age-related differences after ingestion of milk, mainly concerning the volume response and the lipase output. The latter can be explained in the same way as regarding resting conditions. With respect to the volume response, several factors could be implicated, such as the circulating levels of gastrointestinal hormones, the sensitivity of the pancreas to such stimuli, the maturation degree in the secretory mechanisms of the gland or the development of the digestive tract.
在出生后第二、第三和第四周，对15只格拉纳迪纳山羊幼崽的年龄对胰腺外分泌功能的影响进行了研究。这些动物长期插有导管，每天用羊奶人工喂养两次。在摄入牛奶前1小时及摄入后8小时内收集纯胰液。测定胰液流速、碳酸氢盐和氯化物浓度、总蛋白含量及酶活性。在静息状态下，胰液流速随年龄增加而增加，这可能反映了体重和胰腺重量的增加。在出生后第四周，静息淀粉酶和脂肪酶活性显著增加。这可能分别与立即改为高淀粉饮食以及唾液脂肪酶水平同时降低有关。我们的结果还显示，摄入牛奶后存在与年龄相关的差异，主要涉及容量反应和脂肪酶分泌量。后者的解释与静息状态相同。关于容量反应，可能涉及几个因素，如胃肠激素的循环水平、胰腺对这种刺激的敏感性、腺体分泌机制的成熟程度或消化道的发育情况。
