Lopez V, Martínez-Victoria E, Yago M D, Lupiani M J, Mañas M
Institute of Nutrition and Food Technology, Department of Physiology, University of Granada, Spain.
Arch Physiol Biochem. 1997 Apr;105(2):210-5. doi: 10.1076/apab.105.2.210.12922.
A total of 25 preruminant Granadina breed goats were used. They were bottle-fed goat milk ad libitum from postnatal day 3 to 28. Until the age of 3 d, kids were fed colostrum. Body weight, pancreas weight, total protein concentration, enzyme activities in pancreatic tissue and hormone concentrations (cortisol, gastrin, T3 and T4) were determined at 3, 7, 14, 21 and 28 d of age. Our results show that the rates of pancreatic synthesis and secretion of chymotrypsin are well developed at birth in the kid, and may compensate for possible deficiencies in gastric and/or enterocytes intracellular proteolysis. In week 4, there was a marked increase in amylase activity, change that can be attributed to the beginning of the transitional period known as weaning. The significant increase in circulating concentration of cortisol during week 4 suggests the involvement of corticosteroid as a mediator of pancreatic development at weaning. Changes in blood levels of this hormone are believed to be important in the expression of amylase in the neonatal period. However, T3-T4 blood levels remained unchanged from d 3 to 28, suggesting that, in the kid, these hormones appear to have no clear influence upon the postnatal development of the exocrine pancreas.
总共使用了25只反刍前期的格拉纳迪纳品种山羊。从出生后第3天到28天，它们自由采食奶瓶喂养的山羊奶。在3日龄之前，羔羊喂食初乳。在3、7、14、21和28日龄时测定体重、胰腺重量、总蛋白浓度、胰腺组织中的酶活性以及激素浓度（皮质醇、胃泌素、T3和T4）。我们的结果表明，羔羊出生时胰凝乳蛋白酶的胰腺合成和分泌速率发育良好，可能弥补胃和/或肠细胞内蛋白水解的潜在不足。在第4周，淀粉酶活性显著增加，这种变化可归因于称为断奶的过渡期开始。第4周期间循环皮质醇浓度的显著增加表明皮质类固醇作为断奶时胰腺发育的介质发挥作用。据信这种激素血液水平的变化在新生儿期淀粉酶的表达中很重要。然而，从第3天到28天，T3 - T4血液水平保持不变，这表明在羔羊中，这些激素似乎对外分泌胰腺的出生后发育没有明显影响。
