金黄色葡萄球菌dnaA基因的分子克隆与序列分析

Molecular cloning and sequence analysis of the dnaA gene of Staphylococcus aureus.

作者信息

Katayama H, Mizushima T, Miki T, Sekimizu K

机构信息

Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.

出版信息

Biol Pharm Bull. 1997 Jul;20(7):820-2. doi: 10.1248/bpb.20.820.

DOI:10.1248/bpb.20.820

PMID:9255428

Abstract

The dnaA gene of Staphylococcus aureus has now been cloned and sequenced. The deduced amino acid sequence of Staphylococcus aureus DnaA protein is 62% and 39% identical to those of the DnaA protein from Bacillus subtilis and Escherichia coli, respectively. Expression of the dnaA gene of Staphylococcus aureus in Escherichia coli caused lethality in an oriC-dependent manner.

摘要

金黄色葡萄球菌的dnaA基因现已被克隆并测序。推导的金黄色葡萄球菌DnaA蛋白的氨基酸序列与枯草芽孢杆菌和大肠杆菌的DnaA蛋白的氨基酸序列分别有62%和39%的同源性。金黄色葡萄球菌dnaA基因在大肠杆菌中的表达以oriC依赖的方式导致致死性。