López N, Padula P, Rossi C, Miguel S, Edelstein A, Ramírez E, Franze-Fernández M T
Dpto. de Virus, Instituto Nacional de Microbiologia Carlos G. Malbran, Buenos Aires, Argentina.
Virus Res. 1997 Jul;50(1):77-84. doi: 10.1016/s0168-1702(97)00053-1.
Andes virus, one of five hantaviruses known to cause hantavirus pulmonary syndrome (HPS), emerged in 1995 in southwestern Argentina (López et al. (1996) Virology 220, 223-226). The complete nucleotide sequence of Andes virus S genome segment was determined and compared with sequences of viral RNAs in autopsy tissues of more recently reported HPS cases from southwestern Argentina and south of Chile (cases ESQ H-1/96 and CH H-1/96). Andes virus S segment was found to be 1876 nucleotides in length and to encode the nucleocapsid protein (N), 428 amino acids in length. S segment analysis also revealed a long 5' non-coding region (547 nucleotides) which displays three copies of an octanucleotide sequence repeat. Comparisons of S segment sequences of ESQ H-1/96 and CH H-1/96 (82% of the entire genome sequence) with the corresponding sequences of Andes virus revealed identities of 97.2% and 98.5%, respectively. Sequence motifs identical and in the same positions as exhibited in Andes virus 5' non-coding region were found in both, ESQ H-1/96 and CH H-1/96 sequences. Three genome fragments of the M segment sequence of the viruses (representing approximately 34% of the entire sequence) were also analyzed. Comparisons of S and M segment sequences of Andes virus with the corresponding sequences of ESQ H-1/96 showed S and M segment identities which differ by less than 1.4%. Andes virus and CH H-1/96 have S segments that differ by 1.5% from one another while their M segment fragments differ by 5.5-8.2%. Phylogenetic analysis showed that Andes virus along with ESQ H-1/96 and CH H-1/96 form a distinct lineage within the clade containing Bayou and Black Creek Canal viruses. It also showed that Andes virus branch of trees derived from comparisons of S or M sequences differed. It is concluded that Andes virus variants causing HPS circulate east and west of the Andes mountains.
安第斯病毒是已知可导致汉坦病毒肺综合征（HPS）的五种汉坦病毒之一，于1995年在阿根廷西南部出现（洛佩斯等人，《病毒学》，第220卷，第223 - 226页，1996年）。测定了安第斯病毒S基因组片段的完整核苷酸序列，并与来自阿根廷西南部和智利南部最近报告的HPS病例（病例ESQ H - 1/96和CH H - 1/96）尸检组织中的病毒RNA序列进行了比较。发现安第斯病毒S片段长度为1876个核苷酸，编码核衣壳蛋白（N），长度为428个氨基酸。S片段分析还揭示了一个长的5'非编码区（547个核苷酸），其显示出八核苷酸序列重复的三个拷贝。ESQ H - 1/96和CH H - 1/96的S片段序列（占整个基因组序列的82%）与安第斯病毒的相应序列比较，分别显示出97.2%和98.5%的同一性。在ESQ H - 1/96和CH H - 1/96序列中均发现了与安第斯病毒5'非编码区中相同且处于相同位置的序列基序。还分析了病毒M片段序列的三个基因组片段（约占整个序列的34%）。安第斯病毒的S和M片段序列与ESQ H - 1/96的相应序列比较，显示S和M片段同一性差异小于1.4%。安第斯病毒和CH H - 1/96的S片段彼此相差1.5%，而它们的M片段片段相差5.5 - 8.2%。系统发育分析表明，安第斯病毒与ESQ H - 1/96和CH H - 1/96在包含巴尤病毒和黑溪运河病毒的进化枝内形成一个独特的谱系。分析还表明，源自S或M序列比较的树形图中安第斯病毒分支不同。得出的结论是，导致HPS的安第斯病毒变种在安第斯山脉东西两侧传播。
