Meissner John D, Rowe Joan E, Borucki Monica K, St Jeor Stephen C
Department of Microbiology, University of Nevada, FA310/MS200, Reno, NV 89557, USA.
Virus Res. 2002 Oct;89(1):131-43. doi: 10.1016/s0168-1702(02)00129-6.
We have determined the genomic sequence of an Andes virus (ANDV) strain isolated from an infected Oligoryzomys longicaudatus rodent trapped in Chile in 1997. This strain, for which we propose the designation Chile R123, reproduces essential attributes of hantavirus pulmonary syndrome (HPS) when injected intramuscularly into laboratory hamsters (Hooper et al., Virology 289 (2001) 6-14). The L, M, and S segment sequences of Chile R123 are 6562, 3671, and 1871 nt long, respectively, with an overall G+C content of 38.5%. These respective genome segments could encode a 247 kd RNA-dependent RNA polymerase (RdRP), 126 kd glycoprotein precursor (GPC), and 48 kd nucleocapsid (N) protein, in line with other Sigmodontine rodent-associated hantaviruses. Among hantaviruses for which complete genomic sequences are available, Chile R123 is most closely related to Sin Nombre virus (SNV) strain NM R11, with greater than 85% amino acid identity between translated L and S segments and 78% amino acid identity between translated M segments. Because Chile R123 shares essentially 100% amino acid identity in regions of overlap with partially sequenced Argentinian and Chilean ANDV strains, Syrian hamster pathogenicity and the potential for interhuman transmission are features likely common to all ANDV strains.
我们已确定了一株安第斯病毒（ANDV）的基因组序列，该病毒株于1997年从智利捕获的一只感染的长尾稻鼠（Oligoryzomys longicaudatus）中分离得到。我们提议将该病毒株命名为智利R123，当将其肌肉注射到实验室仓鼠体内时，它会重现汉坦病毒肺综合征（HPS）的基本特征（胡珀等人，《病毒学》289（2001）6 - 14）。智利R123的L、M和S基因片段序列分别长6562、3671和1871个核苷酸，总体G + C含量为38.5%。这些各自的基因组片段可分别编码一个247千道尔顿的RNA依赖RNA聚合酶（RdRP）、126千道尔顿的糖蛋白前体（GPC）和48千道尔顿的核衣壳（N）蛋白，这与其他与稻鼠相关的汉坦病毒一致。在已获得完整基因组序列的汉坦病毒中，智利R123与无名病毒（SNV）株NM R11关系最为密切，其翻译后的L和S基因片段之间的氨基酸同一性大于85%，翻译后的M基因片段之间的氨基酸同一性为78%。由于智利R123在与部分测序的阿根廷和智利ANDV株重叠的区域中氨基酸同一性基本为100%，叙利亚仓鼠致病性和人际传播潜力可能是所有ANDV株共有的特征。
