Nishihara H, Miwa H, Watanabe M, Nagashima M, Yagi O, Takamura Y
Department of Applied Biological Resource Sciences, Ibaraki University, Japan.
Biosci Biotechnol Biochem. 1997 Jul;61(7):1067-72. doi: 10.1271/bbb.61.1067.
Random amplified polymorphic DNA (RAPD) analysis was used to discriminate genotypes in five species of Microcystis cyanobacteria. Strains of each group with the identical allozyme genotype (T. Kato et al., Algol. Stud., 1991, 129-140; M. Watanabe, in "Toxic Microcystis," ed. by M.F. Watanabe et al., CRC Press, Tokyo, 1966, pp. 13-34) gave similar RAPD patterns characterizing the respective group. On the other hand, no similarities in RAPD patterns were observed among strains of which allozyme genotypes were different. A good accordance between the RAPD analysis and allozyme divergence indicated a high reliability of both methods for discrimination of the affiliated groups of Microcystis. Several amplified DNA fragments, which were expected to be markers for a particular taxon with identical allozyme genotype, were also observed on the RAPD patterns. Genetic homogeneities of M. novacekii, M. viridis, and M. wesenbergii were shown by RAPD analysis as well as the allozyme genotype. However, significant variations were observed in M. aeruginosa and M. ichthyoblabe in the levels of DNA and proteins (allozymes).
采用随机扩增多态性DNA（RAPD）分析来鉴别五种微囊藻蓝细菌的基因型。每组中具有相同等位酶基因型的菌株（T. Kato等人，《藻类学研究》，1991年，第129 - 140页；M. 渡边，载于M.F. 渡边等人编著的《有毒微囊藻》，CRC出版社，东京，1966年，第13 - 34页）呈现出相似的RAPD图谱，这些图谱表征了各自的组群。另一方面，在等位酶基因型不同的菌株之间未观察到RAPD图谱有相似之处。RAPD分析与等位酶分化之间的良好一致性表明这两种方法在鉴别微囊藻所属组群方面具有较高的可靠性。在RAPD图谱上还观察到几个扩增的DNA片段，它们有望成为具有相同等位酶基因型的特定分类单元的标记。RAPD分析以及等位酶基因型均显示出诺瓦西基微囊藻、绿色微囊藻和韦氏微囊藻的遗传同质性。然而，在铜绿微囊藻和鱼害微囊藻的DNA和蛋白质（等位酶）水平上观察到了显著差异。
