Practical method for the multigram separation of the 5- and 6-isomers of carboxyfluorescein.
作者信息
Rossi F M, Kao J P
机构信息
Medical Biotechnology Center, School of Medicine, University of Maryland, Baltimore 21201, USA.
出版信息
Bioconjug Chem. 1997 Jul-Aug;8(4):495-7. doi: 10.1021/bc970078d.
PMID:9258446
Abstract
An efficient preparative method for separating 5- and 6-carboxyfluorescein is presented. 6-Carboxyfluorescein dipivalate is isolated as its diisopropylamine salt, which can be converted to the free acid or used directly in coupling reactions. The 5-isomer is isolated from the acidified mother liquor. Isomerically pure carboxyfluoresceins are prepared by hydrolysis of the corresponding dipivalates.
摘要
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