Alterations in plasma volume, plasma constituents, renin activity and aldosterone induced by maximal exercise in the horse.

Plasma volume (PV) decreased by 13 per cent following the completion of 1,000 m of maximal exercise in the horse. This study demonstrated that the critical reduction in PV following maximal exercise occurred within 10 mins of completion of exercise, as previously reported in man. Total plasma protein (TPP) increased by 23 per cent at 2 and 5 mins, and by 21 per cent at 10 mins post exercise. Therefore, it does not appear to be an accurate measurement to assess the degree of PV contraction in the horse. Protein was apparently added to the intravascular space either during or following exercise. The changes in osmolality correlated strongly with those in sodium, which is the primary determinant of alterations in plasma tonicity. The increase in osmolality (12 per cent) was similar to the reduction in PV (13 per cent) concluding that a transient hypotonic fluid loss had occurred. The increase in plasma renin activity (PRA) following maximal exercise was followed by an increase in aldosterone (ALD) concentration in both magnitude and time course. Alterations in PV should be considered when interpreting electrolyte and serum enzyme activity data collected following maximal exercise.