Nowatzke W L, Keller E, Koch G, Richardson J P
Department of Chemistry, Indiana University, Bloomington 47405, USA.
J Bacteriol. 1997 Aug;179(16):5238-40. doi: 10.1128/jb.179.16.5238-5240.1997.
The growth of Micrococcus luteus, a soil microorganism that belongs to the high-G+C gram-positive phylogenetic group, is prevented by bicyclomycin, an antibiotic that inhibits the activity of the M. luteus transcription termination factor Rho. A mutant that can grow in 0.3 mM bicyclomycin has a Rho that is insensitive to bicyclomycin and has the single amino acid residue change of Asp474 to Gly. These results indicate that the function of its Rho factor is essential for M. luteus and that growth of a gram-positive organism can be blocked by bicyclomycin.
藤黄微球菌是一种属于高G+C革兰氏阳性系统发育组的土壤微生物，其生长会被双环霉素抑制，双环霉素是一种抑制藤黄微球菌转录终止因子Rho活性的抗生素。一种能在0.3 mM双环霉素中生长的突变体，其Rho对双环霉素不敏感，并且有一个从Asp474到Gly的单氨基酸残基变化。这些结果表明，其Rho因子的功能对藤黄微球菌至关重要，并且革兰氏阳性菌的生长可被双环霉素阻断。
