Xu C F, Chambers J A, Solomon E
Division of Medical and Molecular Genetics, UMDS, 8th Floor, Guy's Tower, Guy's Hospital,London SE1 9RT, United Kingdom.
J Biol Chem. 1997 Aug 22;272(34):20994-7. doi: 10.1074/jbc.272.34.20994.
We have analyzed the promoter region of the human BRCA1 gene in detail and demonstrate that the expression of the BRCA1 gene is under complex regulation. First, its transcription is under the control of two promoters generating two distinct transcripts alpha and beta, and second, promoter alpha is shared with the adjacent NBR2 gene and is bi-directional. Both promoter alpha and promoter beta are responsive to estrogen stimulation. We also discerned that there are striking differences in both the genomic organization and immediate cis-control elements of the BRCA1 gene between humans and mice.
我们详细分析了人类BRCA1基因的启动子区域，并证明BRCA1基因的表达受到复杂调控。首先，其转录受两个启动子控制，产生两种不同的转录本α和β；其次，启动子α与相邻的NBR2基因共享且具有双向性。启动子α和启动子β均对雌激素刺激有反应。我们还发现，人类和小鼠的BRCA1基因在基因组组织和直接顺式调控元件方面存在显著差异。
