Pyshny'i D V, Repkova M N, Lokhov S G, Ivanova E M, Ven'iaminova A G, Zarytova V F
Bioorg Khim. 1997 Jun;23(6):497-504.
The interaction of DNA and RNA with oligodeoxyribonucleotides and their 3'-terminal N-(2-hydroxyethyl)phenazinium derivatives carrying peptide residues with alternating basic and hydrophobic amino acids at the 5'-terminal phosphate was studied. It was found that the introduction of peptide residues (LeuArg)n-Gly-NH2 (n = 2-4) into an oligodeoxyribonucleotide enhances the latter's hybridization ability: each additional LeuArg pair increases the Tm value of the (5')pd(CACACACAAAAAAC).(3')d(TGTGTGTG)p(-LeuArg)n-Gly- NH2 complex by 1.3 degrees C. The reagents did not destort the DNA structure and were capable of site-specific hydrolysis of the phosphodiester bonds of RNA. It was shown that the location of the cleavage sites and the efficacy of the RNA hydrolysis at n = 2 and 4 and at n = 3 strongly differ. The maximum hydrolysis (80%) of tetradecaribonucleotide (5')p(GAUUGAAAAUCCCC) was achieved using peptidyloligodeoxyribonucleotide (3')d(CTAACT)p(LeuArg)4GlyNH2. The possibility of directed cleavage of phosphodiester bonds in tRNAPhe by peptidyloligodeoxyribonucleotides (3')d(CTAACT)p(LeuArg)nGlyNH2 (n = 3 and 4) was shown.
研究了DNA和RNA与寡脱氧核糖核苷酸及其3'-末端携带肽残基的N-(2-羟乙基)吩嗪鎓衍生物的相互作用，这些肽残基在5'-末端磷酸处具有交替的碱性和疏水性氨基酸。发现将肽残基(LeuArg)n-Gly-NH2(n = 2-4)引入寡脱氧核糖核苷酸可增强其杂交能力：每增加一对LeuArg，(5')pd(CACACACAAAAAAC).(3')d(TGTGTGTG)p(-LeuArg)n-Gly-NH2复合物的Tm值增加1.3℃。这些试剂不会扭曲DNA结构，并且能够对RNA的磷酸二酯键进行位点特异性水解。结果表明，在n = 2和4以及n = 3时，切割位点的位置和RNA水解的效率有很大差异。使用肽基寡脱氧核糖核苷酸(3')d(CTAACT)p(LeuArg)4GlyNH2可实现对十四聚核糖核苷酸(5')p(GAUUGAAAAUCCCC)的最大水解(80%)。研究表明，肽基寡脱氧核糖核苷酸(3')d(CTAACT)p(LeuArg)nGlyNH2(n = 3和4)能够对苯丙氨酸tRNA中的磷酸二酯键进行定向切割。
