Mironova Nadezhda L, Pyshnyi Dmitrii V, Stadler Dmitry V, Prokudin Ivan V, Boutorine Yuri I, Ivanova Eugenia M, Zenkova Marina A, Gross Hans J, Vlassov Valentin V
Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentiev Avenue 8, Russian Federation, Novosibirsk, 630090.
J Biomol Struct Dyn. 2006 Jun;23(6):591-602. doi: 10.1080/07391102.2006.10507084.
Artificial ribonucleases, conjugates of short oligodeoxyribonucleotides and peptides built of arginine, leucine, proline, and serine, were synthesized and assessed in terms of ribonuclease activity and specificity of RNA cleavage. A specific group of the conjugates was identified that display T1-ribonuclease-like activity and cleave RNA predominantly at G-X sequences. Circular dichroism study of the structures of the most active conjugates, free peptide (LR)4G, and oligonucleotides revealed that conjugation of oligonucleotide to the peptide results in a specific peptide folding that possibly provides ribonuclease activity to the conjugate.
合成了人工核糖核酸酶，即由精氨酸、亮氨酸、脯氨酸和丝氨酸构成的短寡脱氧核糖核苷酸与肽的缀合物，并对其核糖核酸酶活性和RNA切割特异性进行了评估。鉴定出了一组特定的缀合物，它们表现出类似T1 - 核糖核酸酶的活性，主要在G - X序列处切割RNA。对活性最高的缀合物、游离肽(LR)4G和寡核苷酸的结构进行圆二色性研究表明，寡核苷酸与肽的缀合导致特定的肽折叠，这可能为缀合物提供核糖核酸酶活性。
