Nygren P A, Uhlén M
Department of Biochemistry and Biotechnology, Royal Institute of Technology (KTH), Stockholm, Sweden.
Curr Opin Struct Biol. 1997 Aug;7(4):463-9. doi: 10.1016/s0959-440x(97)80108-x.
The combination of combinational protein chemistry and powerful selection techniques has resulted in the development of novel protein ligands based on the randomization of surface residues of a parental protein which is used as a scaffold. Such binding proteins, selected from libraries via specific binding towards a given target ligand, have the potential to replace natural antibodies in various biotechnological applications.
组合蛋白质化学与强大的筛选技术相结合，已基于用作支架的亲本蛋白质表面残基的随机化开发出新型蛋白质配体。此类通过对给定靶标配体的特异性结合从文库中筛选出的结合蛋白，有潜力在各种生物技术应用中取代天然抗体。
