Not T, Città A, Lucchesi A, Torre G, Martelossi S, Ventura A
Clinica Pediatrica, Istituto per l'Infanzia, Trieste, Italy.
Eur J Pediatr. 1997 Aug;156(8):616-8. doi: 10.1007/s004310050676.
Anti-endomysium antibody (AEA) was evaluated in 136 subjects by indirect immunofluorescence using both cryosections of monkey oesophagus (MO) and the human umbilical cord vein (HUCV) as substrate. This human tissue gave results comparable to those of MO. In particular, the HUCV sections showed positive results in all 22 newly diagnosed cases of coeliac disease. Compared to the MO sections, the sensitivity, specificity and positive predictive values of HUCV tissue was 100%.
HUCV tissue can replace MO for AEA detection and can make the screening for coeliac disease easier both in at-risk and in normal populations, with a remarkable saving, both in terms of money and of monkeys.
采用间接免疫荧光法，以猴食管冷冻切片（MO）和人脐静脉（HUCV）为底物，对136名受试者的抗肌内膜抗体（AEA）进行了评估。这种人体组织得出的结果与猴食管冷冻切片相当。特别是，在所有22例新诊断的乳糜泻病例中，人脐静脉切片均显示阳性结果。与人脐静脉切片相比，人脐静脉组织的敏感性、特异性和阳性预测值均为100%。
人脐静脉组织可替代猴食管冷冻切片用于抗肌内膜抗体检测，在高危人群和正常人群中均可使乳糜泻的筛查更加简便，在金钱和猴子数量方面均有显著节省。
