Rouppe van der Voort J N, van Zandvoort P, van Eck H J, Folkertsma R T, Hutten R C, Draaistra J, Gommers F J, Jacobsen E, Helder J, Bakker J
Graduate School of Experimental Plant Sciences, Wageningen Agricultural University, Department of Nematology, The Netherlands.
Mol Gen Genet. 1997 Jul;255(4):438-47. doi: 10.1007/s004380050516.
The allele specificity of AFLP markers was assessed in five relatively unrelated potato genotypes. To this end, two diploid mapping populations of potato, F1SH x RH and F1AM x RH, were analysed using four and six AFLP primer combinations, respectively, recently applied to the analysis of the genetically well characterized backcross population BC_C x E. The AFLP profiles of the five parents revealed 733 AFLP markers and, when identical primer combinations were used, 131 comigrating AFLP markers were identified. After construction of five parental maps, the genomic positions of these comigrating AFLP markers were compared and 117 markers (89%) which targeted the same genomic region were assumed to be homologous. Of these putative homologues, 20 markers, each cloned from at least two genotypes, were sequenced and 19 sets of amplification products were shown to be nearly identical. The number of AFLP markers previously mapped in population BC_C x E ranged from three to eleven per chromosome, which allowed a reliable assessment of chromosome numbers from individual linkage groups obtained in populations F1SH x RH and F1AM x RH. The high incidence of corresponding AFLP alleles was confirmed by using an additional set of five primer combinations. The 733 AFLP markers localized provide a valuable reference collection for future mapping studies in potato. As a consequence AFLP analysis may replace more laborious locus-specific marker techniques.
在五个亲缘关系相对较远的马铃薯基因型中评估了AFLP标记的等位基因特异性。为此，分别使用四组和六组AFLP引物组合对两个马铃薯二倍体作图群体F1SH×RH和F1AM×RH进行了分析，这些引物组合最近被用于对遗传特征明确的回交群体BC_C×E的分析。五个亲本的AFLP图谱显示了733个AFLP标记，当使用相同的引物组合时，鉴定出131个共迁移的AFLP标记。构建五个亲本图谱后，比较了这些共迁移AFLP标记的基因组位置，假定117个（89%）靶向相同基因组区域的标记是同源的。在这些假定的同源物中，对每个从至少两个基因型中克隆的20个标记进行了测序，结果显示19组扩增产物几乎相同。先前在群体BC_C×E中定位的AFLP标记数量在每条染色体上从三个到十一个不等，这使得能够可靠地评估从群体F1SH×RH和F1AM×RH中获得的各个连锁群的染色体数量。通过使用另外一组五组引物组合，证实了相应AFLP等位基因的高发生率。所定位的733个AFLP标记为未来马铃薯的作图研究提供了有价值的参考集合。因此，AFLP分析可能会取代更费力的位点特异性标记技术。
