Li Q G, Lindman K, Wadell G
Department of Virology, Umeå University, Sweden.
Arch Virol. 1997;142(7):1307-22. doi: 10.1007/s007050050162.
The complete nucleotide sequence and the predicted amino acid sequence of the adenovirus type 7 hexon gene were determined. The hydropathy of the hexon proteins from human adenovirus types 2, 3, 4, 5, 7, 12, 16, 40, 41, and 48, bovine adenovirus type 3, murine adenovirus type 1, and avian adenovirus types 1 and 10 was analysed. The presence of purines and pyrimidines in the second position of the codons was correlated to hydrophilicity and hydrophobicity, respectively. Comparison of the hydrophilicity plots of eight hexons showed seven hypervariable regions to be distributed on the surface. A large portion of the hypervariable regions manifests hydrophilicity. The strength of the surface charge accumulated on the hydrophilic and hydrophobic regions correlated to the tissue tropism of the different adenovirus types. Analysis of codon usage for adenovirus hexons showed that among synonymous codons those with cytidine in the third position were preferably used to a great extent. Analysis of the nucleotide and amino acid sequence pair distances and the phylogenetic tree of 14 hexon proteins showed members of subgenera B, D and E to be closely related, especially Ad4 and Ad16, and subgenus A to be closely related to subgenus F.
测定了7型腺病毒六邻体基因的完整核苷酸序列和预测的氨基酸序列。分析了人2型、3型、4型、5型、7型、12型、16型、40型、41型和48型腺病毒、牛3型腺病毒、鼠1型腺病毒以及禽1型和10型腺病毒六邻体蛋白的亲水性。密码子第二位嘌呤和嘧啶的存在分别与亲水性和疏水性相关。八种六邻体亲水性图谱的比较显示，七个高变区分布在表面。高变区的很大一部分表现出亲水性。亲水和疏水区域积累的表面电荷强度与不同腺病毒类型的组织嗜性相关。腺病毒六邻体密码子使用情况分析表明，在同义密码子中，第三位为胞嘧啶的密码子在很大程度上被优先使用。对14种六邻体蛋白的核苷酸和氨基酸序列对距离以及系统发育树的分析表明，B、D和E亚属的成员密切相关，尤其是Ad4和Ad16，A亚属与F亚属密切相关。
