Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Witten/Herdecke University, 58453 Witten, Germany.
Department of Prosthodontics, School of Dentistry, Faculty of Health, Witten/Herdecke University, 58455 Witten, Germany.
Int J Mol Sci. 2021 Sep 17;22(18):10075. doi: 10.3390/ijms221810075.
Previous studies reported on the broad-spectrum antiviral function of heparin. Here we investigated the antiviral function of magnesium-modified heparin and found that modified heparin displayed a significantly enhanced antiviral function against human adenovirus (HAdV) in immortalized and primary cells. Nuclear magnetic resonance analyses revealed a conformational change of heparin when complexed with magnesium. To broadly explore this discovery, we tested the antiviral function of modified heparin against herpes simplex virus type 1 (HSV-1) and found that the replication of HSV-1 was even further decreased compared to aciclovir. Moreover, we investigated the antiviral effect against the new severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) and measured a 55-fold decreased viral load in the supernatant of infected cells associated with a 38-fold decrease in virus growth. The advantage of our modified heparin is an increased antiviral effect compared to regular heparin.
先前的研究报道了肝素的广谱抗病毒功能。在这里，我们研究了镁修饰肝素的抗病毒功能，发现修饰后的肝素对永生化细胞和原代细胞中的人腺病毒 (HAdV) 具有显著增强的抗病毒作用。核磁共振分析显示，肝素与镁结合时构象发生变化。为了广泛探索这一发现，我们测试了修饰肝素对单纯疱疹病毒 1 型 (HSV-1) 的抗病毒功能，发现与阿昔洛韦相比，HSV-1 的复制进一步减少。此外，我们研究了对新型严重急性呼吸综合征冠状病毒 2 型 (SARS-CoV-2) 的抗病毒作用，并测量了感染细胞上清液中病毒载量降低了 55 倍，病毒生长降低了 38 倍。与普通肝素相比，我们修饰肝素的优势在于抗病毒效果增强。
