Sreeramulu D, Nair K M, Qadri S S, Rao K V, Sivakumar B
National Institute of Nutrition, Indian Council of Medical Research, Jamai Osmania PO, Hyderabad, India.
Ann Nutr Metab. 1997;41(2):126-36. doi: 10.1159/000177988.
Different modes of iron depletion and repletion were studied in monkeys to understand the sequential changes in and the relative importance of different biochemical indicators of iron status. Six control monkeys were divided into two groups, one was fed an iron-deficient diet (group 1) and the other underwent phlebotomy in addition to receiving an iron-deficient diet (group 2). Previously iron-depleted monkeys were subdivided into 4 groups of 3 animals each. While one group was continued on the iron-deficient diet (group 3), the second group received parenteral iron (group 4), the third group (group 5) received a sufficient-iron-containing diet, and the fourth group was fed 50% of the iron requirement. All indicators of iron status like hemoglobin (Hb), erythrocyte protoporphyrin (EPP), serum transferrin saturation and serum ferritin were monitored periodically, in addition to liver and bone marrow iron. all the indicators except serum ferritin and liver iron showed a decrease in group 2. On the other hand, animals receiving parenteral iron (group 4) showed an increase in all the parameters except serum ferritin. The dietary supplementation produced an increase in Hb and a decrease in EPP only (groups 5 and 6). There was a significant positive correlation between changes in bone marrow iron and Hb concentration depending on the severity of depletion and repletion. Both serum ferritin and liver iron did not respond to changes in dietary iron. Another parameter which responded to repletion was EPP. Serum ferritin and liver iron did not respond to changes in dietary iron or was not sensitive to subclinical iron deficiency. The results indicate that change in Hb is more sensitive to detect the deficiency of iron. It was also observed that different parameters respond variably under different modes of depletion and repletion.
为了解铁状态不同生化指标的序贯变化及其相对重要性，对猴子进行了不同的铁耗竭和补充模式研究。6只对照猴子被分为两组，一组喂食缺铁饮食（第1组），另一组除接受缺铁饮食外还进行放血（第2组）。先前已缺铁的猴子被再细分为4组，每组3只动物。一组继续喂食缺铁饮食（第3组），第二组接受胃肠外铁剂（第4组），第三组（第5组）接受含铁充足的饮食，第四组喂食铁需求量的50%。除了肝脏和骨髓铁之外，还定期监测所有铁状态指标，如血红蛋白（Hb）、红细胞原卟啉（EPP）、血清转铁蛋白饱和度和血清铁蛋白。除血清铁蛋白和肝脏铁外，所有指标在第2组均下降。另一方面，接受胃肠外铁剂的动物（第4组）除血清铁蛋白外所有参数均升高。饮食补充仅使Hb升高而EPP降低（第5组和第6组）。根据耗竭和补充的严重程度，骨髓铁变化与Hb浓度之间存在显著正相关。血清铁蛋白和肝脏铁均未对饮食铁的变化作出反应。另一个对补充有反应的参数是EPP。血清铁蛋白和肝脏铁未对饮食铁的变化作出反应，或对亚临床缺铁不敏感。结果表明，Hb变化对检测铁缺乏更敏感。还观察到不同参数在不同的耗竭和补充模式下反应各异。
