Myers J S, Gomes J A, Siepser S B, Rapuano C J, Eagle R C, Thom S B
Wills Eye University Hospital, Philadelphia, Pennsylvania, USA.
J Refract Surg. 1997 Jul-Aug;13(4):356-61. doi: 10.3928/1081-597X-19970701-11.
Transforming growth factor beta (TGF-beta) has been shown to affect wound repair. Anti-transforming growth factor beta 1 antibodies have been shown to neutralize its activity.
Seventeen New Zealand White rabbits underwent unilateral photorefractive keratectomy followed by corneal staining with dichlorotriazinyl fluorescein. Three groups received topical transforming growth factor beta 1: 1 microgram/ml, 10 micrograms/ml, and 100 micrograms/ml; one group topical anti-transforming growth factor beta antibody (200 micrograms/ml); and a control group vehicle only. Corneal haze was graded from 0 to 4, weekly. Rabbits were sacrificed at 5 weeks and histopathological analysis and fluorescence microscopy performed.
All treated eyes developed haze and had epithelial erosions. No statistically significant differences in haze score were seen among individual treatment groups (Kruskal Wallis p > 0.05). The anti-transforming growth factor beta antibody group had less haze than all other groups at every week after the first. Comparing all transforming growth factor beta 1 treated eyes as one group to the antibody group, significantly less haze was seen at weeks 3 and 4 in the antibody treated group (p = 0.028 and 0.013, respectively). This study is limited by small group size and further studies are needed to confirm these results.
TGF-beta may be involved in stromal haze formation, and topical anti-TGF-beta 1 antibody may help reduce the development of stromal haze.
已证实转化生长因子β（TGF-β）会影响伤口修复。抗转化生长因子β1抗体已被证明可中和其活性。
17只新西兰白兔接受单侧准分子激光原位角膜磨镶术，随后用二氯三嗪基荧光素进行角膜染色。三组分别接受局部应用转化生长因子β1：1微克/毫升、10微克/毫升和100微克/毫升；一组局部应用抗转化生长因子β抗体（200微克/毫升）；对照组仅使用赋形剂。每周对角膜混浊进行0至4级评分。5周后处死兔子并进行组织病理学分析和荧光显微镜检查。
所有治疗的眼睛均出现混浊并有上皮糜烂。各治疗组之间在混浊评分上无统计学显著差异（Kruskal Wallis检验p>0.05）。抗转化生长因子β抗体组在首次给药后的每周混浊程度均低于所有其他组。将所有接受转化生长因子β1治疗的眼睛作为一组与抗体组进行比较，抗体治疗组在第3周和第4周的混浊程度明显较低（p分别为0.028和0.013）。本研究受样本量小的限制，需要进一步研究来证实这些结果。
TGF-β可能参与基质混浊的形成，局部应用抗TGF-β1抗体可能有助于减少基质混浊的发展。
